Final concentration of BSA BSA stock (uL) Buffer (uL) Absorbance (mg/ml) (540 nm) in protein assay 2 1.5 1 1.1 0.75 0.7 0.5 0.3 0.25 0.1

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter28: Atomic Spectroscopy
Section: Chapter Questions
Problem 28.13QAP
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26. You are provided with a stock solution - 2 mg/ml BSA, calculate and fill in the blanks in the table
below for preparation of the solutions (final volume is 1 mL); and draw a standard curve.
Final concentration of BSA
BSA stock (uL)
Buffer (ul)
Absorbance
(mg/ml)
(540 nm) in
protein assay
2
1.5
1
1.1
0.75
0.7
0.5
0.3
0.25
0.1
Transcribed Image Text:26. You are provided with a stock solution - 2 mg/ml BSA, calculate and fill in the blanks in the table below for preparation of the solutions (final volume is 1 mL); and draw a standard curve. Final concentration of BSA BSA stock (uL) Buffer (ul) Absorbance (mg/ml) (540 nm) in protein assay 2 1.5 1 1.1 0.75 0.7 0.5 0.3 0.25 0.1
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