For the binding graph of Protein B with ligand X on the right, (i) Estimate the K, for Protein B with ligand X. Explain how you determined the K. (ii) Protein B binds to ligand Z with a Kg = 4.8 x 10+. Display a curve on the graph to represent Protein B binding to ligand Z. Using the information you have, explain which ligand Protein B has higher affinity for.

Appl Of Ms Excel In Analytical Chemistry
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ISBN:9781285686691
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Chapter12: Spectrochemical Methods
Section: Chapter Questions
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For the binding graph of Protein B with ligand X on the
right, (i) Estimate the K, for Protein B with ligand X.
Explain how you determined the Ka. (ii) Protein B
binds to ligand Z with a Ka = 4.8 x 106. Display a curve
on the graph to represent Protein B binding to ligand Z.
Using the information you have, explain which ligand
Protein B has higher affinity for.
0.8
0.6
0.4
0.2
0 1 2 3 4 5 6 7 8 9 10
[Ligand] umol L
An uncatalyzed reaction has AG = 59.5 kJ mol and a rate of reaction that is 6.31 x 109 umol s1 at 25.0 °C
and pH 8.1. When an enzyme is added, the rate of reaction is 1.49 umol s' at 25.0 °C and pH 8.1.
Calculate the activation energy of the catalyzed reaction.
Transcribed Image Text:For the binding graph of Protein B with ligand X on the right, (i) Estimate the K, for Protein B with ligand X. Explain how you determined the Ka. (ii) Protein B binds to ligand Z with a Ka = 4.8 x 106. Display a curve on the graph to represent Protein B binding to ligand Z. Using the information you have, explain which ligand Protein B has higher affinity for. 0.8 0.6 0.4 0.2 0 1 2 3 4 5 6 7 8 9 10 [Ligand] umol L An uncatalyzed reaction has AG = 59.5 kJ mol and a rate of reaction that is 6.31 x 109 umol s1 at 25.0 °C and pH 8.1. When an enzyme is added, the rate of reaction is 1.49 umol s' at 25.0 °C and pH 8.1. Calculate the activation energy of the catalyzed reaction.
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