A protein binds a ligand with a ka value of 7.4 x 104 M-1s-1 and a kd value of 6.4 x 10-4 s-1. What is the KD value of this binding event in units of nM? Please report your answer to two significant figures and include units.

A protein binds a ligand with a ka value of 7.4 x 104 M-1s-1 and a kd value of 6.4 x 10-4 s-1. What is the KD value of this binding event in units of nM? Please report your answer to two significant figures and include units.

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter11: Dynamic Electrochemistry

Section: Chapter Questions

Problem 8P

See similar textbooks

Related questions

Q: How many moles of Mg are there in a sample of Mg that contains 1.36x1024 atoms? moles

A: Given Atoms of Mg = 1.36 x 1024 atoms

Q: 1. 2. 3. 1. 3. 3. 2. M NaHSo 18.5 second lowing ermining the Rate Law for a Chemical Reaction ata Sh...

Q: 2. Assign priorities to the groups on this chiral carbon. Label it as R or S. QH H - ÓCH3

A: We have to assign the priorities to the groups on the chiral carbon and label it as R or S.

Q: Choose a balanced chemical equation for the reaction of molecular oxygen with beryllium metal to for...

Q: When calibrating a pH meter, 3 (three) standard color-coded buffer solutions are used, which of thes...

A: The correct answer about PH calibration is given below

Q: HO In reference to chiral carbon A, what is the configuration of the structure? (A)S B)R

A: Answer is given below:-

Q: What is the vapor pressure at 100 degree celsius of a solution containing 1.68 g of sucrose (MW 342....

A: The relative lowering of vapour pressure is equal to the mole fraction of solute present in the solu...

Q: omplete the table below for calculating the molar mass of the compound sulfur tetrafluoride. Molar m...

A: Given , Sulfur tetrafluoride

Q: At present, automobile batteries are sealed. When lead storage batteries discharge, they produce hyd...

A: Molar mass of hydrogen = 1.00794 g/mol Molar mass of H2 = 1.00794 × 2 = 2.01588 gram/mole Given Mas...

Q: e boiling point of

A: Since you have asked multiple questions, we will solve the first question for you. If you want any s...

Q: Read carefully the given partial names in IUPAC alkane nomenclature and check the columns where they...

Q: Calculate the cell potential of the cell: Use Nernst Equation and Debye Huckel Law Pb(s)PbBr2(s) | C...

A: Nernst equation is the equation for determining the cell potential for a given cell for a particular...

Q: 14. Draw two complete curved arrow mechanisms that accounts for both the products shown in the react...

A: Alkyl shift: An alkyl shift occurs when a carbocation does not contain a hydrogen atom that is prese...

Q: What is observed when the Na2SO3 solution is added to the CuCl2 solution?

A: Given question is : What is observed when the Na2SO3 solution is added to the CuCl2 solution = ?

Q: Sodium hypochlorite (NaOCl, the active ingredient of all bleaches) was dissolved in a solution buffe...

A: pH of buffer solution is calculated by using Henderson Hassel balch equation

Q: Balance the given molecular equation using redox (electron-transfer method). Identify the element ox...

Q: 1. How many MOLES of dinitrogen tetroxide are present in 4.67 grams of this compound ? moles. 2. How...

A: Mole of substance is defined as given mass divided by its molar mass.

Q: CALCULATE: 1. The numbwr of moles of 0.020 kg H20 2. The number of moles of 8.30 x 10²⁰ molecules...

Q: sa dipole moment?

Q: Consider the following reaction. C12H22O11 + 12O2 → 12CO2 + 11H2O + 1342kcal How many grams of suc...

A: Here we have to calculate the mass of sucrose needed to undergo combustion to produce 2546 KCal of e...

Q: When magnesium reacts with nitrogen, the reaction container becomes very hot. The ΔH for this reacti...

Q: Which of the following HC is most soluble in water? A) Benzene and derivatives B Alkyne C) Alkane D)...

A: The correct option is option A.

Q: Read carefully the given partial names in IUPAC alkane nomenclature and check the columns where they...

A: answer :

Q: The scientific methods is applied to natural phenomena. What is natural phenomena? Give two examples...

Q: Define combustion. Select all that apply. O The rapid reaction of a substance with oxygen. O The rap...

Q: 2. What is the mass of KHP that you will weigh? a. The instructions read "Weigh out accurately 700 t...

A: answer :

Q: Supposing compound A is 2-methylbutane-2,3-diol and compound B is n-pentane. Compound A is more solu...

A: Water is a polar solvent and it can dissolve an ionic or polar covalent compound. The solubility of ...

Q: Complete the table below for calculating the molar mass of the ionic compound chromium(II) sulfide. ...

A: Given, Chromium (II) sulfide.

Q: A certain reaction is second order in N, and first order in H,. Use this information to complete the...

A: Given Order of reaction with respect to N2 = 2 Order of reaction with respect to H2 = 1 [ N2 ...

Q: 3) Show the product(s) & intermediates for the following reactions.

A: In the above reactions the possible products and intermediate are given below.

Q: 12. Provide one other contributing resonance form for structures A, B, and C. Show all electron move...

A: Resonance structures are formed by movement of pair of electron or single electron.

Q: Complete the table below for calculating the molar mass of the ionic compound chromium(III) sulfide ...

A: A compound is formed by the electrostatic interaction between the cation and the anion. The cation i...

Q: 3. How many lb mol of NaNO3 are there in 100 lb?

Q: Show the calculations on how to prepare 2.5 L of 1.5 M buffer with pH=10.0 from CH3NH3Cl and CH3NH2....

A: Here we have determine how to prepare 2.5 L of 1.5 M buffer solution with pH= 10 from CH3NH3Cl and C...

Q: 3. A typical diet in the United States provides 15% of the calories from protein, 45% from carbohydr...

A: We know that., 1 gram of Fat contain = 9 Kcal1 gram of proteins contain = 4 Kcal1 gram of Carbohydr...

Q: The absorbance spectra for X3+ and Y2+ overlap significantly. X3+* forms a red-green complex in solu...

A: A question based on tools for analytical chemistry that is to be accomplished.

Q: Some measurements of the initial rate of a certain reaction are given in the table below. [N:] H2|in...

Q: pka E

Q: Calculate the lambda max of the given compound using Woodward-Fieser Rules. R AcO Select one: O 303 ...

A: Woodward–Fieser rules: The Woodward–Fieser rules are used for the calculation of the wavelength of t...

Q: The 3D image below is that of an allylic carbocation intermediate formed by the protonation of a con...

A: The addition of HBr to conjugated dienes can lead to 1,2 (Kinetic product) or 1,4 addition products ...

Q: %. The percent by mass of bromine in PNBR2 is (Enter your answer to four significant figures.)

Q: Determine the point group of the following orbital:

Q: Why is light of at least a certain frequency needed to cause the photoelectric effect? O A certain q...

A: Photoelectric effect :- J.J Thomson observed that when a photon of having certain frequency strikes ...

Q: rue or False 1. Sample preparation of infrared spectroscopy uses KBr as the blank because Kbr has 10...

A: Ans.1. Sample preparation of infrared spectroscopy uses KBr as the blank because KBr has 100% transm...

Q: Which statement is correct? I. The carbons in a chair conformation will have two axial or two equato...

Q: 1.08 g H2 is allowed to react with 9.83 g N2 , producing 1.36 g NH3 . Part A What is the theoretical...

Q: e equilibrium constant, Kp, for the following reaction is 0.497 at 500 K: 디s(g) PCI3(9) + Cl2(g) lcu...

A: Kp is equilibrium constant

Q: A tertiary amine is more basic compared to a secondary amine. (A) True B False

A: Amines are Lewis base due to the presence of a lone pair of electrons on the nitrogen atom of amine.

Q: 5. Organic molecules are still subjected to general principles of inorganic chemistry. a. True b. Fa...

A: Generally organic molecules contain C,H,O,N atoms.

Q: 11 A 14 0 L tank contains 250g of methane (CH4) gas at 27 atm at 298 K. Accidentally, 190 g of CO2 w...

A: Given Capacity of the tank = 14.0 L Weight of methane = 250 g Pressure of methane in tank = 27 at...

Question

100%

A protein binds a ligand with a ka value of 7.4 x 10⁴ M^-1s^-1 and a kd value of 6.4 x 10^-4 s^-1. What is the KD value of this binding event in units of nM? Please report your answer to two significant figures and include units.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1: Protein binding

VIEW

Step 2: KD for protein binding

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Metals in Transport, Tranfer and Transcription

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

A protein binds a ligand with a KD value of 0.25 M. If you are at a ligand concentration of 0.63 M, what is the fraction bound? Please report your answer to two significant figures. There should be no units in your answer
A dimeric binding protein has a KD1 of 50 nM and a Hill coefficient of 1.4. What is the fraction of the protein that has ligand bound at 17 nM concentration of ligand?
19. The association constant of protein X for ligand Y is: 100 micromolar. At what concentration of ligand is half fo the protein bound?
From the following binding data of a ligand binding to a protein, which set of the K50 and Hill Coefficient is the most likely? [Ligand](M) 0.1 Y 0.2 0.2 0.5 0.4 0.8 0.6 0.9 0.2 M, 1 0.04 M, 2 0.008 M, 3 0.45 M, 0.5
I did not understand soultion for the question.. The relationship between Affinity and Association & Dissociation Constant. Four proteins (A-D) all bind the same ligand (X), with different affinities. For protein A & B we know that they have a binding site for X with a Kd (dissociation constant) of 10⁻⁵and 10⁻⁸ M, respectively. For protein C and D we know that they have a binding site for ligand X with a Ka (association constant) of 10³and 10⁵M, respectively. Which protein has the highest affinity for ligand ? Explain your reasoning. How do you make them all in same constant so that values can be compared and the one with highest Ka or lowest Kd (Highest affinity in both cases) can be ruled out.
You prepare a solution of protein and its ligand where the initial concentrations are [Protein] = 10 nM and [Ligand] = 10 nM. At equilibrium you measure the [PL] = 5 nM. If the protein - ligand association can be represented by: P + L <--> PL What is the dissociation constant (Kd) of the protein for the ligand under the solvent conditions you have chosen? a. 1 nM b. 0.2 nM-1 c. 5 nM d. 10 nM
Relationship between Affinity and Association & Dissociation Constant. Four proteins (A-D) all bind the same ligand (X), with different affinities. For protein A & B we know that they have a binding site for X with a Kd (dissociation constant) of 10-5and 10-8M, respectively. For protein C and D we know that they have a binding site for ligand X with a Ka (association constant) of 103and 105M, respectively. Which protein has the highest affinity for ligand X? Explain your reasoning.
what is the Ligand receptor interaction prediction of the structure in the picture that i attached thank you
Predict whether the following complexes would show Jahn-Teller distortion: Ammonium pentachlorooxidochromate(V), meff = 1.8 mB Potassium hexaiodidomanganate(IV), meff = 3.8 mB Potassium hexachoridocuprate(II), meff = 1.8 mB Hexaaquamanganese(II) chloride, meff = 6.0 mB
If the association constant, Ka, for a certain protein-ligand pair is 50,000, what is the dissociation constant, Kd? Please provide your answer in decimal form, not scientific notation.
Some Protein P binds to 1 molecule of X. The fractional binding curve (θ vs. [Ligand]) shows that θ = ⅓ when [X] = 6 mM. What is the Kd for X binding to P?
INORGANIC Chemistry Starting product was [(Me5dien)CoCl2] and reacted with NaSCN to produce [(Me5dien)Co(SCN)2] Using the graphs attached, the IR data tells you that the binding mode is N coordination. How does the IR data agree (or disagree) with the peak shifts (Uv-Vis spect) IR Product 1, [(Me5dien)CoCl2] 29041.04, 2812.76, 2761.62 cm-1 (carbon bond) 1457.35 cm-1 (CH2) 1263.91 cm-1 (possibly C-N stretch) Product 2, [(Me5dien)Co(SCN)2] 2968.54-2870.67 cm-1 (C-C) 2059.41 cm-1 (N=C=S) 1470.28 cm-1 (CH2) Uv-vis [(Me5dien)CoCl2] Peak A: 531.8 nm, 0.722 Abs Peak B: 609.7 nm, 0.666 Abs [(Me5dien)Co(SCN)2] Peak A: 510.20 nm, 0.357 Abs Peak B: 608.20 nm, 0.401 Abs