Question

From a kinetics experiment, the Vmax was determined to be 450µM∙min-1.  For the kinetic assay, 0.1mL of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 125,000 g/mole.  Assume a reaction volume of 700µL.  Calculate the kcat  (in sec-1) for the enzyme.

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