QUESTION 38In a 250µl reaction containing 0.3 nmol of a Michaelis enzyme with Km = 4.4 x 10 M and saturatingsubstrate concentration, product was formed at an initial velocity of 6.3 x 10 M min-1. What is kcat forthis enzyme? Give your answer in 3 sigfigs in scientific notation, for example 1.20 e-3Click Save and Submit to save and submit. Click Save All Answers to save all answers.O Type here to search78°F Mostly cloudyEnd4)DIIPrtScnF8HomeF9F6F2F3F4*-&78.4.#3

Given, Vo= 6.3 * 10-3 M min-1 Km= 4.4 * 10-5 M [S]= 1 (Because its given that the condition is…

Answered: In a 250ul reaction containing 0.3 nmol…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 22 When the final product of a series of enzymatically-catalyzed reactions binds to the first enzyme in the pathway to limit its production, it generally uses ___ because the structure of this final product is generally not similar to that of any of the enzyme's normal substrates. Allosteric activation Zymogen activation Covalent modification Competitive inhibition Allosteric inhibition
Question 37 Locard’s exchange principle states that, whenever two objects come into contact with one another, there is exchange of materials between them. a. True b. False
Question 1: When the CAC is run in reverse by microorganisms that use it to fix carbon, the citrate synthase reaction is different and is catalyzed by an enzyme called ATP-citrate lyase. Write the reaction catalyzed by ATP-citrate lyase, then briefly (in one sentence) explain why the use of different chemistry (different from the ‘normal’ direction) makes sense here.
Question:- The enzyme aromatase is found in the cytoplasm of some cells and converts testosterone to estrogen. You decide to test aromatase from a particular cell, and oops, your lab partner admits he drastically increased the pH in all the test tubes. Which of the following is a likely result? a. The enzyme will be denatured and the substrate will not bind to the active site. b. The enzyme will convert testosterone to estrogen at a faster rate. c. The mistake will have no effect on the experiment, because enzymes are not sensitive to pH. d. The free energy will be lowered and the reaction will not proceed spontaneously.
Question 21>In 1668, Francesco Redi performed a series of experiments on spontaneous generation. He began by putting similar pieces of meat into eight identical jars. Four jars were left open to the air, and four were sealed. He then did the same experiment with one variation: Instead of sealing four of the jars completely, he covered them with gauze (the gauze excluded the flies while allowing the meat to be exposed to air). In both experiments, he monitored the jars and recorded whether or not maggots (young flies) appeared in the meat.What hypothesis was being tested in the initial experiment with open versus sealed jars?A.The type of meat used affects the likelihood of spontaneous generation.B.Spontaneous generation can occur only if meat is exposed to air.C.Spontaneous generation is more likely during the long days of summer.D.Maggots do not arise spontaneously, but from eggs laid by adult flies.
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In a 250ul reaction containing 0.3 nmol of a Michaelis enzyme with Km = 4.4 x 10 M and saturating substrate concentration, product was formed at an initial velocity of 6.3 x 10³ M min-1. What is kcat for this enzyme? Give your answer in 3 sigfigs in scientific notation, for example 1.20 e