Consider an enzyme (P) that gets activated by forming a dimer (P2): 2P P2 At 25 °C, we have AH- 19 kJ/mol and AS - 65 kJ/mol, for this dimerization reaction, Identify the correct statement.
Q: Given Tagatose, Briefly explain its expected reaction (based on their structural formula) to the…
A: Tagatose is ketohexose i.e. it has 6 carbons and is a ketose sugar.
Q: Make a concept map covering about the following: a. SYPHILIS b. Anti-Streptolysin O Test (ASO TEST)…
A: Hi! Thank you for the question. We are authorized to answer three subparts at a time, since you have…
Q: What is substrate-level phosphorylation? 2. Although oxygen does not participate directly in the…
A: ATP is the energy currency of the cell and is praoduct of catabolic pathways that is used in…
Q: In a double-stranded DNA molecule, how are the sequences of each strand related to each other? A…
A: DNA are the nucleotide which contains genetic information in our body and are found in nucleus.
Q: Given a part of DNA undergoing replication. Copy and write the corresponding bases in the new…
A: Semiconservative mode of DNA replication describes the mechanism of DNA replication in all living…
Q: Look at tho structuros of tho blood group dotorminante. Red blood cell N-Acetyl- D-glucosamine Blood…
A: Blood group antigens are O-glycoproteins, N-glycoproteins, and glycolipids that are present on the…
Q: For the electron transport chain, all are inhibitors except: Select one: O a. Antimycin A O b.…
A: Most of the free energy released during the oxidation of glucose to carbon dioxide is retained in…
Q: . What mRNA base sequence would be obtained from the following portion of a gene?
A: Genetic information is transferred from genes to the proteins via messenger RNA.…
Q: Explain the mechanism of Warburg effect and how it benefits cancer cells
A: Cancer means uncontrolled cell growth. This uncontrolled cell growth may cause a lump of cell or…
Q: After intense activity, your muscles feel sore because of ______. Group of answer choices the…
A: During intense activity our body needs more energy (ATP).
Q: Which statement regarding glycolysis is TRUE? Group of answer choices Both substrate-level…
A: Glycolysis is the process of oxidation of one mole of glucose into two moles of pyruvate. It occurs…
Q: Explain the correlation between fasting and gluconeogenesis in terms of the hormone released by the…
A: Gluconeogenesis is the process of Synthesis of glucose from non Carbohydrate sources like…
Q: The "D" in DNA stands for which of the following?
A: DNA : Chemical name for molecule which carries genetic instructions in the living organisms.
Q: You are required to isolate, purify and verify the purity of an enzyme from cytosol of a plant
A: Enzymes are usually composed of proteins which is specific for its substrate. It has immense…
Q: 4. How does compromised pyruvate kinase activity lead to anemia ?
A: Anemia is a disorder in which the body does not have enough healthy red blood cells to transport…
Q: Which of the following enzyme catalyzes the first step of glycolysis? Group of answer choices…
A: Glycolysis : Process in which the glucose gets broken to produce pyruvate, ATP, NADH and water.
Q: 12. You can distinguish epinephrine hydrotartrate from norepinephrine hydrotartrate by: A. Water…
A: Hi, thanks a lot for submitting the multiple questions. As we are allowed to answer one question at…
Q: Explain which of the following substances ATP, CoA-SH, FAD and NAD+ have the subunits in their…
A: Four substances ATP, CoA-AH, FAD and NAD+ are given. Here, we have to identify the subunits in their…
Q: Fill out the table below. Determine whether the rate of the metabolic pathways will increase or…
A: Regulation of blood glucose is largely done by endocrine hormones , through a negetive feedback…
Q: In cholesterol, to which ring of the steroid system is the hydroxyl group attached? B O A
A: cholesterol is a sterol and is the primary compound from which synthesis of many steroid hormones,…
Q: well-conserved regions in multiple sequence aligmements: a. are areas of structural importance b.…
A: Multiple Sequence Alignment (MSA) is the alignment of three or more comparable length biological…
Q: 2. Compound 1 below is metabolized to compound 2 by CYP. The enzyme is gradually inactivated during…
A: Cytochrome P450 are a class of proteins with the ability to catalyze oxidation reactions. They…
Q: Which types of ion exchange resin will the the peptide Ala-Glu-lle-Lys- Leu-Asp-Gly bind to at the…
A: Ion exchange chromatography consists of column with loaded resin that can exchange oppositely…
Q: What 3 antibiotics that are active against G= and G- organisms contain both proteinogenic and…
A: Hi, thanks a lot for submitting the question. Here question have typo error and G= is G+ I believe…
Q: RNA DNA Strand Sugar residue Nitrogenous bases Main Function
A: Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA) are the two types of nucleic acids that…
Q: Hello! Can you help me in determining the statements below if it's true or false? It's all about…
A: Lipids are organic compounds that are insoluble in water and soluble in organic solvents like ether…
Q: Question 5 CH3(CH2)14–Ċ-0–CH2-(CH2)28-CH3 Palmitic acid Triacontanol
A: Lipids are a class of biomolecules that are mostly dissolved in organic solvent and contains a…
Q: Polysaccharides may gel through a variety of different mechanisms. Which of these statements is…
A: Carbohydrates are divided into 3 classes monosaccharides, disaccharides, and polysaccharides.…
Q: The circulatory system is a O closed network of arteries, veins, and capillaries O an open network…
A: Circulatory system is one of the important systems of the body involved in various processes like,…
Q: The table below summarizes the results for Xanthoproteic test. Provide the correct remarks from the…
A: Proteins are big, complex molecules that serve a number of important tasks in the human body. They…
Q: Why water is polar while carbon dioxide is not?
A: Polar molecules are the molecules with different poles with two different charges. The two different…
Q: 1. Is the Homo sapiens phenylalanine hydroxylase (PAH) gene encoding a non-coding protein or an…
A: Gene is a portion of the genome that can be transcribed or a functional unit of the genome…
Q: What part of the protein sequence leads to different functions
A: Proteins are peptides , composed of monomers of polymers,Protein function is directly related to the…
Q: How does compromised pyruvate kinase activity lead to anemia?
A: "Since you have posted multiple question we will answer the first question for you. If you want any…
Q: Some enzymes can be inhibited by high concentrations of their substrates. I expression for the rate…
A: In the biological systems , enzymes acts as catalysts . Enzyme help to accelerate the reactions.…
Q: Does SARS-CoV-2 conform to the central dogma of molecular biology that was coined by Francis Crick?…
A: Introduction: SARS-Cov-2 is a member of a large family of viruses called coronavirus disease 19…
Q: In the Biuret Assay for protein concentration determination, the role of sodium potassium tartrate…
A: The biuret test is a chemical test that can be performed to determine whether an analyte has peptide…
Q: Classify the following amino acids base on their side chain if it's either -Non-polar aliphatic R…
A: Amino acids are the molecules that contain an amino group and a carboxylic group linked through the…
Q: Choose the wrong statement: Select one: O a. Energy can be converted from one form to another. O b.…
A: ATP (adenosine triphosphate) is a molecule that provides energy to drive many processes in the cell,…
Q: A protein sample of unknown concentration was placed in a cuvette with a path length of 1 cm and the…
A: The concentration of a protein is directly proportional to the amount of light it absorbs. The…
Q: Identify chemical reactions involved in food preparation and preservation. Explain how these…
A: The oldest method of food preservation includes drying, refrigeration and fermentation…
Q: Using specific examples, compare and contrast G-protein coupled receptors and nuclear receptors with…
A: The binding of signaling molecules to specific receptors and production of a cellular response…
Q: With Fehling's reagent (under certain conditions) interact: A. Glucose B. Quinine hydrochloride C.…
A: Fehling's reagent is a reagent commonly employed in differentiation of water soluble carbohydrates…
Q: How does NH4SO4 affect water structure? What does this have to do with protein solubility? I thought…
A: The solubility of a protein in solution depends on nature of the protein and the concentration of…
Q: D. PCR of lacZ GENE 1. You will be preparing a PCR reaction for your "G" and "L" tubes. When…
A: Given Values: The total volume of the PCR reaction tube = 50 μl The total volume of the master mi…
Q: 5. Convert each of the following 3' to 5' DNA sequences to 5' to 3' DNA sequences. a. 3' ATCG 5' b.…
A: DNA contains all the genetic information of an organism in the form of genes. These genes are…
Q: You are working in a factory that is producing chemicals from the bacterium Corynebacterium…
A: Corynebacterium glutamicum is a facultative anaerobic gram-positive, catalase-positive, rod-shaped…
Q: Choose the wrong statement: Select one: O a. Competitive inhibitors competing with the substrate O…
A: Enzymes are the biological catalysts, that increase the rate of a chemical reaction. The enzymes may…
Q: In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Solid…
A: Okazaki fragments are short stretches of DNA on the lagging strand, which is synthesized in the…
Q: 2. ( To the right is a schematic diagram of His the active site in the Michaelis complex of a-chy-…
A: Chymotrypsin is a protease that cleaves a peptide at the C-terminal of all aromatic amino acid.…
Step by step
Solved in 2 steps
- Why can’t an enzyme have a kcat/KM value greater than 109 M−1 ∙ s−1?Consider the following chemical equation whose delta(G) = 9kcal/mol: AC + BD ---> AB + CD what are the reactants and what are the products is this reaction spontaneous? How do you know? Is energy released or consumed by this reaction? If an enzyme, which catalyzes this reaction is added, what will happen to delta (G) If this reaction is coupled to another reaction, whose delta(G) is -12 kcal/mol, what will be the net delta(G) value? will the overall reaction be spontaneouswhat is the purpose of staggering the start and stop of the reactions? With reference to your experimental protocol, what is the purpose of staggering the start and stop of the reactions? A.To ensure that the reaction occurs with different amounts of enzyme in each tube so as to ensure comparability between reaction tubes. B.To ensure that the reaction occurs at exactly the same pH in each tube so as to ensure comparability between reaction tubes. C.To ensure that the reaction occurs for exactly the same time interval (30 minutes) in each tube so as to ensure comparability between reaction tubes. D.To ensure that the reaction occurs with exactly the same amount of substrate in each tube so as to ensure comparability between reaction tubes.
- You begin to study enzyme Z, which catalyzes a simple reversible reaction that interconverts compound S and compound P. You observe that the ∆G´° for the S to P conversion to be –6 kJ/mol, and that compound S has ∆G´° for binding to enzyme Z of –15 kJ/mol, while compound P has a ∆G´° for binding to enzyme Z of –13 kJ/mol. Please explain the effect of enzyme Z on conversion of S to P. (Your answer should include a graph qualitatively showing energy versus reaction progress; however, you still need to explain youranswer in words!) not sure how to make the correct graph.Consider the analogy of the jiggling box containing coins that was described on page 85. The reaction, the flipping of coins that either face heads up (h) or tails up (T), is described by the equation h ↔ T, where the rate of the forward reaction equals the rate of the reverse reaction.a. What are ΔG and ΔG° in this analogy? b. What corresponds to the temperature at which the reaction proceeds? What corresponds to the activation energy of the reaction? assume you have an “enzyme,” called jigglase, which catalyzes this reaction. What would the effect of jigglase be and what, mechanically, might jigglase do in this analogy?How many moles of active sites are there in 1 mg of enzyme? Assume that each subunit has one active site.
- An enzyme catalysed reaction has a Km of 8 mM and a Vmax of 13 nM.s-1. Use the Michaelis-Menten equation to calculate the reaction velocity when the substrate concentration is 18 mM.The following question focuses on how the parameters regulating enzyme function might change, and how these might appear graphically on a Michaelis-Menten plot and a Lineweaver-Burke plot. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2 + H20 > H+ + HCO3 There are many different isoforms of this enzyme. (see for instance http://en.wikipedia.org/wiki/Carbonic_anhydrase 1 Assume that one variant has a Km of 10 µM and a different variant has a Km of 100 µM. Draw on the same graph a typical Michaelis-Menton plot showing the alteration in the rate of carbonic anhydrase as the CO2 level is varied for the two different variants of enzyme, assuming the concentration of the enzyme (10 mM) in the test tube is kept constant. Assume that you have equal amounts of the two different variants of carbonic anhydrase in a number of test tubes and that the Vmax for both enzymes are the same. Be sure to label the axes. For the same conditions as above, draw a…Assume you have an enzyme that catalyzes a reaction that breaks down dopachrome. At t = 0 s, the absorbance at 475 nm is 0.2 when you add the enzyme. At t = 30 s, would you expect the absorbance to be less than or greater than 0.2?
- Consider the following data for an enzyme-catalyzedhydrolysis reaction in the presence and absence ofinhibitor I: Using a Michaelis-Menten plot, determine Km for theuninhibited reaction and the inhibited reaction.The following reaction coordinate diagram charts the energy of a substrate molecule (S) as it passes through a transition state (X‡) on its way to becoming a stable product (P) alone or in the presence of one of two different enzymes (E1 and E2). How does the addition of either enzyme affect the change in Gibbs free energy (ΔG) for the reaction? Which of the two enzymes binds with greater affinity to the substrate? Which enzyme better stabilizes the transition state? Which enzyme functions as a better catalyst?A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction The researchers begin to characterize the enzyme. (a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 uM/s. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.) (b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 uM, the researchers find that V0 = 300 nM/s. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.) (c) Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 uM/s, and the measured Km in (b) is now 15 uM. Based on this information, can you figure out what type of inhibitor is ANGER? (Use table 6.9 at the end of the…