Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700
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- If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints, and dust prior to reading it in the spectrophotometer, how might the absorbance value of the sample be affected? (Higher, Lower, No Change) Explainif the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?Discuss the types of interferences in atomic absorption spectrophotometry.
- What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?
- What is the main application of Atomic absorption spectrophotometer (AAS)?What are the processes of concentrate samples analysis in Uv-visible spectroscopy? Please shortly answer at your own words(3-4 lines).If a circular object or specimen extends halfway across the scanning field, what isits diameter in millimeters? ___________
- Explain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is developed to 10 cm. How far apart in centimeters are the spots for the two compounds?A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.