Given this sample data: 200 colonies on the LB plate + ampicillin plate Calculate the number of transformed cells per milliliter of the transformation cell mixture. Assume that 100 microliters of the transformation mix was plated on each plate. Select one: O a. 20 cells O b. 200 cells O c. 2,000 cells O d. 20,000 cells
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- A.Why do you plate the cells from the viable count on LB agar without ampicillin? B.If you observe 100 colonies on your 1/100 plate, how many colonies do you expect if everything works perfectly on your 1/1000 plate?Place the following steps for setting up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour the molten agarose into the gel cast . D. Load ladder and DNA samples into the well . E. Assemble the gel cast including comb . F. Place lid on the gel running apparatus . G. Remove the comb and add running buffer. H. Orient the gel in the gel running apparatus . I. Add Sybr-safe to the molten agarose. J. Connect the leads to both the gel running apparatus and the power pack .You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.
- Please answer both asap If there were only 2 tests for COVID-19 and Test A has a high sensitivity but low specificity while Test B has low sensitivity and high specificity, which test would you choose and why? -A Covid-19 diagnostic test has a sensitivity of 80%. What would be the percentage of false negatives for the test?Please answer fast What are some similarities and differences in Bradford assay and Nanodrop (to obtain direct absorbance measurements). What are the benefits and setbacks of them. How is 260/280 absorbance ratio significant?Make an illustration showing the steps in the Goldbeater’s test.
- What is the Kirby-Bauer Test? Describe it and explain how you would interpret the results.Looking at the image of results of an experiment: A. What Hypothesis is being tested in the experiment B. What Assay is used to test the Hypothesis in A? (Use a diagram) C. What result would you expect to see if the Hypothesis in A is true? And to what degree does the data support the hypothesis?Increasing the tube voltage on a clinical x-ray unit: a. Improves the resolution of an x-ray image. b. Increases the intensity of the Characteristic x-rays. c. Increases the intensity of the Bremsstrahlung x-rays. d. Increases the energy of the Bremsstrahlung x-rays. e. Increases the energy of the Characteristic x-rays. and why??
- Can you please answer question 5. According to your answers provide at question 2 there should be a certain number of bands visible on your gel. Suppo. you did this practical and you observed less bands on your gel, give possit I explanations for this.A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?When Griffith injected mice with IIR strain mixed with heat-killed IIIS bacteria, A. The mice survived, and he recovered live type IIIR cells. B. The mice survived, and he recovered live type IIS cells. C. The mice died, but he recovered live type IIIR cells. D. The mice died, but he recovered live type IIS cells. E. The mice died, but he recovered live type IIIS cells.