This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed? Test Tube Concentration (uM) Absorbance @ 600nm #1 0 0.00 #2 5 0.090 #3 10 0.187 #4 20 0.368 #5 40 0.715 #6 60 1.075     Table 3. Succinate Dehydrogenase Assay @ 600 nm Time point (min): Resuspended Pellet Reaction Absorbance Supernatant Reaction Absorbance Enzyme Reaction Control Absorbance 0 43.2 44.2 46.1 3 43.3 44.8   6 43.0 44.9   9 42.6 45.2   12 43.5 45.6   15 44.0 45.7   18 43.8 45.9   21 44.0 46.4   24 44.1 46.5   27 43.9 46.7   30 43.7 46.9 43.6

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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This is my data collected from a Succinate Dehyrogenase Enzyme Assay - I am asked to plot a Concentration vs Time while plotting the Resuspended and Supernatant Reaction; how can this be completed?

Test Tube

Concentration (uM)

Absorbance @ 600nm

#1

0

0.00

#2

5

0.090

#3

10

0.187

#4

20

0.368

#5

40

0.715

#6

60

1.075

 

 

Table 3. Succinate Dehydrogenase Assay @ 600 nm

Time point (min):

Resuspended Pellet Reaction

Absorbance

Supernatant Reaction Absorbance

Enzyme Reaction Control Absorbance

0

43.2

44.2

46.1

3

43.3

44.8

 

6

43.0

44.9

 

9

42.6

45.2

 

12

43.5

45.6

 

15

44.0

45.7

 

18

43.8

45.9

 

21

44.0

46.4

 

24

44.1

46.5

 

27

43.9

46.7

 

30

43.7

46.9

43.6

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