Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over the course of the run? A)Increase the pH of the mobile phase. B)Decrease the pH of the mobile phase. C)Increase the vapor pressure of the analytes by increasing the temperature. D)Increase mobile phase polarity by increasing the fraction of polar solvent in a binary mobile phase. E)Decrease mobile phase polarity by mixing with a less polar solvent to better compete with the nonpolar stationaryphase. Plz explain properly why statement is not correct
Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over the course of the run? A)Increase the pH of the mobile phase. B)Decrease the pH of the mobile phase. C)Increase the vapor pressure of the analytes by increasing the temperature. D)Increase mobile phase polarity by increasing the fraction of polar solvent in a binary mobile phase. E)Decrease mobile phase polarity by mixing with a less polar solvent to better compete with the nonpolar stationaryphase. Plz explain properly why statement is not correct
Chapter14: Chromatography
Section: Chapter Questions
Problem 1P
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Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over the course of the run?
A)Increase the pH of the mobile phase.
B)Decrease the pH of the mobile phase.
C)Increase the vapor pressure of the analytes by increasing the temperature.
D)Increase mobile phase polarity by increasing the fraction of polar solvent in a binary mobile phase.
E)Decrease mobile phase polarity by mixing with a less polar solvent to better compete with the nonpolar stationaryphase.
Plz explain properly why statement is not correct
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