Below is overlaid chromatograms for the separation of cytidine on a bare silica stationary phase using a90/10 acetonitrile/0.1 M ammonium formate buffer. Explain the observed pH dependence of theretention time.pH 3.3pH 4.0pH 6.422.22.42.62.83.23.43.6Time (min)

The time period for which a solute is present in the column for the interaction with the stationary…

Answered: Below is overlaid chromatograms for the…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.2QAP

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Similar questions

Ion exchange chromatography consists of? i. stationary phase with a matrix of charged ionizable functional groups ii. mobile phase consisting of a buffer at a certain pH iii. coloured fluorescent dye iv. analyte that requires an optimum wavelength A. ii and iii B. i and iv C. iii and iv D. i and ii
In color chromatography of plant pigments, what complications would a dried-out column (solvent level is below the top of the silica) introduce to the elution and isolation of pigments?
Which statements are true for molecular exclusion chromatography?I Molecular exclusion chromatography separates on the basis of size.II Small molecules can fit in the pores of the stationary phase and effectively pass through a larger volume, and elute last.III The pores in the stationary phase are too small for large molecules to pass through, so large molecules elute first.IV Molecular exclusion chromatography with a hydrophilic stationary phase and an aqueous solvent is called gel permeation chromatography. A. I, II, III B. III and IV C. II only D. I and II only E. I, III, and IV

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Below is overlaid chromatograms for the separation of cytidine on a bare silica stationary phase using a 90/10 acetonitrile/0.1 M ammonium formate buffer. Explain the observed pH dependence of the retention time. pH 3.3 pH 4.0 pH 6.4 2.2 2.4 2.6 2.8 3.2 3.4 3.6 Time (min)