Hexokinase catalyzes the following reaction in glycolysis: Glucose + ATP-- glucose-6-phosphate + ADP The standard free-energy change for this reaction in the direction written is -16.7 kJ/mol. The concentrations of the related reaction components in the hepatocyte of a mammalian cell are: Glucose: 5 mM Glucose-6-phosphate: 0.083 mM ATP: 1.85 mM ADP: 0.14 mM What is the actual free-energy change for the reaction at body temperature (37 C)? -14.3 kJ/mol -33.3 kJ/mol +33.3 kJ/mol +14.3 kJ/mol

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.5: Blood Glucose And Hemoglobin A1c
Problem 8RQ
icon
Related questions
Question
100%

Can someone help with this question?

 

Hexokinase catalyzes the following reaction in glycolysis:
Glucose + ATP -→
• glucose-6-phosphate + ADP
The standard free-energy change for this reaction in the direction written is -16.7 kJ/mol.
The concentrations of the related reaction components in the hepatocyte of a mammalian cell are:
Glucose: 5 mM
Glucose-6-phosphate: 0.083 mM
ATP: 1.85 mM
ADP: 0.14 mM
What is the actual free-energy change for the reaction at body temperature (37 C)?
-14.3 kJ/mol
-33.3 kJ/mol
+33.3 kJ/mol
+14.3 kJ/mol
Transcribed Image Text:Hexokinase catalyzes the following reaction in glycolysis: Glucose + ATP -→ • glucose-6-phosphate + ADP The standard free-energy change for this reaction in the direction written is -16.7 kJ/mol. The concentrations of the related reaction components in the hepatocyte of a mammalian cell are: Glucose: 5 mM Glucose-6-phosphate: 0.083 mM ATP: 1.85 mM ADP: 0.14 mM What is the actual free-energy change for the reaction at body temperature (37 C)? -14.3 kJ/mol -33.3 kJ/mol +33.3 kJ/mol +14.3 kJ/mol
Use the following table for all questions in the problem set related to glycolysis:
TABLE 18.1 Reactions and Thermodynamics of Glycolysis
AG"
AG
Reaction
Enzyme
(k/mol)
at 25°c
(kJ/mol)
Hexokinase
Glucokinase
a-D-Glucose + ATPt-=
-16.7
850
-33.9*
glucose-6-phosphate?- + ADP- + H*
Glucose-6 phosphate²-=fructose-6-phosphate-
Fructose-6-phosphate- + ATP-=
fructose-1,6-bisphosphate- + ADP- + H*
Fructose-1,6-bisphosphate
dihydroxyacetone-P- + glyceraldehyde-3-P:-
Dihydroxyacetone-P=glyceraldehyde-3-P-
Glyceraldehyde-3-P- + P?- + NAD*=
1,3-bisphosphoglycerate- + NADH + H*
1,3-Bisphosphoglycerate + ADP-
Phosphoglucoisomerase
Phosphofructokinase
+1.67
0.51
-2.92
-14.2
310
-18.8
Fructose bisphosphate
aldolase
+23.9
6.43 X 10-3
-0.23
Triose phosphate isomerase
Glyceraldehyde-3-P
dehydrogenase
Phosphoglycerate kinase
+7.56
0.0472
+2.41
+6.30
0.0786
-1.29
-18.9
2060
+0.1
3-Pglycerate- + ATP-
3-Phosphoglycerate =2phosphoglycerate
2-Phosphoglycerate-=
Phosphoglycerate mutase
+4.4
0.169
+0.83
Enolase
+1.8
0.483
+1.1
phosphoenolpyruvate-+ H,0
Phosphoenolpyruvate- + ADP- + H* =
Pyruvate + ATpt-
Pyruvate + NADH + H*= lactate" + NAD*
Pyruvate kinase
-31.7
3.63 x 10
-23.0
Lactate dehydrogenase
-25.2
2.63 x 10
-14.8
Transcribed Image Text:Use the following table for all questions in the problem set related to glycolysis: TABLE 18.1 Reactions and Thermodynamics of Glycolysis AG" AG Reaction Enzyme (k/mol) at 25°c (kJ/mol) Hexokinase Glucokinase a-D-Glucose + ATPt-= -16.7 850 -33.9* glucose-6-phosphate?- + ADP- + H* Glucose-6 phosphate²-=fructose-6-phosphate- Fructose-6-phosphate- + ATP-= fructose-1,6-bisphosphate- + ADP- + H* Fructose-1,6-bisphosphate dihydroxyacetone-P- + glyceraldehyde-3-P:- Dihydroxyacetone-P=glyceraldehyde-3-P- Glyceraldehyde-3-P- + P?- + NAD*= 1,3-bisphosphoglycerate- + NADH + H* 1,3-Bisphosphoglycerate + ADP- Phosphoglucoisomerase Phosphofructokinase +1.67 0.51 -2.92 -14.2 310 -18.8 Fructose bisphosphate aldolase +23.9 6.43 X 10-3 -0.23 Triose phosphate isomerase Glyceraldehyde-3-P dehydrogenase Phosphoglycerate kinase +7.56 0.0472 +2.41 +6.30 0.0786 -1.29 -18.9 2060 +0.1 3-Pglycerate- + ATP- 3-Phosphoglycerate =2phosphoglycerate 2-Phosphoglycerate-= Phosphoglycerate mutase +4.4 0.169 +0.83 Enolase +1.8 0.483 +1.1 phosphoenolpyruvate-+ H,0 Phosphoenolpyruvate- + ADP- + H* = Pyruvate + ATpt- Pyruvate + NADH + H*= lactate" + NAD* Pyruvate kinase -31.7 3.63 x 10 -23.0 Lactate dehydrogenase -25.2 2.63 x 10 -14.8
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Medical terminologies
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Basic Clinical Laboratory Techniques 6E
Basic Clinical Laboratory Techniques 6E
Biology
ISBN:
9781133893943
Author:
ESTRIDGE
Publisher:
Cengage