How does the ONPG/MUG test allow a sample to be assayed simultaneously for the presence of both total coliforms and E. coli?

Q: What is an example of an experimental approach for determining whether a beef burger contains a food…

A: Beef burgers served rare or undercooked may contain harmful bacteria that could cause food…

Q: What would you surmise if you examined a Kirby-Bauer assay and found individual colonies of the…

A: Antimicrobial is the efficacy to kill the microbes. Drugs that have antimicrobial properties are…

Q: How does pasteurization affect the number of bacteria present in milk? What differences, if any,…

A: To control microbial growth or contamination there are a number of aseptic techniques developed that…

Q: fluorogenic methods that can be used in detecting coliforms and E.coli

A:

Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli

A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…

Q: Explain how are the physiological characteristics of bacteria identified using biochemical test?

A: The physiological properties that are used for the identification of bacteria includes the nutrient…

Q: Why would not the size of the inhibition in the Kirby-Bauer test directly relate to the comparative…

A: In Kirby–Bauer testing, discs containing antibiotics are placed on agar where bacteria are growing,…

Q: What will happen if the protein that you are assaying using zymography is not fixed properly using…

A: Zymogram analysis is done in order to and characterize collagenases, metalloproteinases, and other…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Biochemical pathway will be as-  MM- MM+C - MM+A - MM+B - CM Hint- the more the positive signs of…

Q: What is the logic behind testing for E. coli to detect fecalcontamination of water?

A: Bacteria are unicellular prokaryotic organism that maintains a definite shape since it contains the…

Q: If coliforms were present on a plated sample, would you know with certainty that the coliforms are…

A: Coliform microbes are characterized as rod-shaped, gram-negative, non-spore-forming, and…

Q: Identify two other methods of protein content determination and differentiate them from the Bradford…

A: Proteins are the biomolecules that are made up of Aminoacids. Protein estimation is very important…

Q: Based on the same two strains of E. coli from the previous question, why do you see growth in the…

A: Ampicillin is a penicillin type of antibiotic substances containing medium So the  strain II can…

Q: How could Qualitative Analysis of Proteins Tests be an essential practical or real-life application…

A: Proteins are large biological molecules composed of amino acids, amino acids contain both amino…

Q: Why is protein testing in human samples important?

A: The protein test measures the total amount of two classes of proteins that are found in the blood.…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Auxotrophic microorganisms cannot produce a certain organic chemical essential for their growth. The…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: Why is CFU more applicable to a culture of Streptococcus than to a culture of E. coli?

A: Colony-forming unit (CFU) represents the number of colonies formed on the media plate from one…

Q: n not more than 100 words, What antigens/strains are used for the Weil-Felix test, and from what…

A: Well Felix test is an agglutination test for the diagnosis of Rickettsia infection. The Well Felix…

Q: Name five functional traits of microorganisms. What dose the functional diversity describe? Do we…

A: Functional traits of the microbes describe individually expressed phenotypes or characteristics of…

Q: Is bile esculin test a efficient test for unknown intestinal bacteria?

A: In the intestine, only those bacteria that are not killed by the bile survive. The normal bacteria…

Q: can the phenol red test also be used to determine if a certain bacterium can metabolze various…

A: Differential media is a type of growth media used in the labs to grow and differentiate or…

Q: If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what that…

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: The isolation of Salmonella Typhimurium and S.Typhi require different protocols: A) In what aspects…

A: Salmonella Typhimurium and salmonella typhi are the 2 diffrent specius of bacteria under same genus…

Q: Five different strains of E. coli (1, 2, 3, 4 and 5) were streaked onto a series of tester plates.…

A: Having a minimal medium decides what exactly is used by the bacteria that is under study for its…

Q: Which chemotherapeutic agent was the most effective and least effective to each test organism (E.…

A: chemotherapeutic agent is a substance used to kill or inhibit the growth of cancer cells.…

Q: Among these protein assays, which is appropriate for solutions with high protein concentration or…

A: Protein assay is a quantitative analytical technique. It is carried out to determine the amount of…

Q: Outline the strategies commonly used to detect for pBR322 in E.coli

A: Plasmids are DNA molecules that exist outside of the chromosomes. They are microscopic, round, and…

Q: What biochemical tests did we use to distinguish staphylococci from streptococci?

A: Staphylococcus and streptococcus both occur in cocci shape and both are gram positive organisms.…

Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…

A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…

Q: How does one can differentiate E.coli from Proteus vulgaris

A: Proteus vulgaris and E. coli, both are gram negative in nature and be allied to same family…

Q: In a Triple Sugar Iron test, what are the results and (what should they look like) for the bacteria:…

A: triple sugar ion test or TSI is a confirmatory as well as a differentiative biochemical test which…

Q: Once the completed test establishes the presence of coliforms in the water sample, why might you…

A: Water is tasteless, transparent, inorganic. Colorless, and odorless chemical substance. Its chemical…

Q: What happens if you dont' add maltose in the molten top agar when performing an plaque assay? What…

A: Introduction  Plaque assay is a microbiology technique performed to quantify the viral…

Q: How you will detect covid-19 using ELISA?

A: ELISA is an Enzyme-Linked Immunosorbent Assay, which is used to detect antibodies or antigens in the…

Q: Do the results from a confirmed coliform test represent total or faecal coliform and why?

A: Fecal coliform bacteria may occur in ambient water as a result of the overflow of domestic sewage or…

Q: E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar…

A: MacConkeys Agar is a selective and differential culture medium. It specifically select Gram negative…

Q: What kinds of organisms may be plentiful in a milk sample, yet give a negative reductase test?

A: Methylene blue reduction test is based on the principle of oxygen reduction by increased microbial…

Q: How are methods of precipitating proteins, such as heat and treatment with alcohol, also successful…

A: Yes they are successful in killing harmful microorganisms..  Precipitation is explain as protein is…

Q: Why is Muller Hinton Agar (MHA) ideal for antibiotic susceptibility testing

A: MHA(Mueller Hinton Agar) was developed by Mueller and Hinton in 1941. It is used to test antibiotic…

Q: What is the difference between the total and faecal coliform

A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…

Q: If the five-digit code that is tabulated cannot be found in the EnteroPluri-Test Codebook, what…

A: Introduction: EnteroPluri-Test is an identification system that offers complete identification of…

Q: Name three reasons why the ELISA test is useful in detecting microbes

A: ELISA:            ELISA ~ Enzyme-Linked Immunosorbent Assay                   A screening tool used…

Q: IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR…

A: Biuret test of protein is to done to quantify the proteins or to detect the concentration of…

Q: Why rapid tests are not the gold standard in Covid19 detection. Explain.

A: Diagnosis of the Sar-Cov-2 virus is usually done by means of isolating samples collected from the…

Q: You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture.…

A: Answer : First step of the kirby-Bauer test :-

Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…

A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.