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If coliforms were present on a plated sample, would you know with certainty that the coliforms are disease-causing? Why or why not?
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- If you did the multiple-tube technique, list one advantage and one disadvantage of each method of detecting coliforms?What is the coliform test when is it used? What is its purpose? How does it function? What are the advantages and disadvantages of utilizing the coliform test?Why is water tested for coliform bacteria rather than for pathogenic bacteria which may be present?
- What would be the expected out come of a Nitrate reduction test If the bacteria is a pathogenic intestinal bacteria?Do the results from a confirmed coliform test represent total or faecal coliform and why?What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria?
- can the phenol red test also be used to determine if a certain bacterium can metabolze various carbohydrates?In performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?You have retrieved your MHA (Mueller-Hinton agar) plate, your materials, and your E. coli culture. What is the first step to prepare your Kirby-Bauer test?
- If coliform bacteria are native to human colons, why the big concern over coliform contamination?How are methods of precipitating proteins, such as heat and treatment with alcohol, also successful in killing harmful microorganisms?What are the characteristics of an effective indicator organism? Write down some examples. Suppose you filtered a sample and found the Fecal Coliform count to be 20 CFU/100ml but the Total Coliform count was 0 CFU/100ml, do you think this result is correct? If so, why/why not?