If you perform the motility test on Motility Medium and hanging drop for Bacillus cereus and B. subtilis, what will you see?

Q: What is the purpose of the alcohol (OH) in the gram staining process?

A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…

Q: How might storage of the coconut water samples at room temperature after collection and before…

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…

A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: How would you draw out a step in an experiment that included the following? Please help, I am so…

A: Horizontal gene transfer (HGT) is largely responsible worldwide for increasing the incidence of…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: In spread plate method, colonies form within the agar and agar surface. In pour plate method,…

A: Introduction Agar media is essential for the study of microorganisms and molecular biology, It is a…

Q: Gram stain of Nodule Suspension Describe the morphology of the crushed nodules under microscopes…

A: The bacteria are stained with the gram stain and can be divided into two groups on the basis of the…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: What is the difference between the pour plate method and the spread plate method? Why might you…

A: Standard plate count method is a growth based approach that counts the number of living…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: What concentration of agar is used in a semisolid medium for motility determination?

A: Motility is a feature characteristic of only some bacteria. This ability allows the bacteria to move…

Q: A Gram stain indicates purple cells that are round and attached in chains. Which of the following…

A: Gram staining is a simple technique, in which two categories of bacteria are identified based on…

Q: What concentration of agar is used in a semisolid medium for motility determination? How does that…

A: Agar is a polysaccharide that is obtained from marine seaweeds. Agar is mostly used in food and…

Q: Why is it necessary to use a 24-hrold culture in gram staining?

A: Note: Since you have posted multiple independent questions in the same request, we will solve the…

Q: Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 10…

A: Cell count: The cell count data is generally utilized to predict the number of living microbes…

Q: Determine the number of bacterial cells per ml. in the original culture. 1ml 1ml 1ml 1ml 99ml 99ml…

A: Serial dilutions are used to minimize bacterial concentrations to a level necessary for a certain…

Q: Why do we use the term CFU (colony forming unit) instead of reporting bacteria per ml? Explain.

A: The  bacterial species are responsible for causing several diseases within the human body. So, for…

Q: Why do you think the continuous culture has higher chances of contamination than batch and fed-batch…

A: Continuous culture may be a set of techniques wont to reproducibly cultivate microorganisms at…

Q: You were given a mixed nutrient agar broth culture of bacteria  1a. How will you determine the…

A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…

Q: The students of a Microbiology class were tasked to transfer or subculture a pure culture of…

A:

Q: What is the correct shape of the unknown bacterium growing on your mannitol salt agar

A: Mannitol salt agar also known as MSA is commonly used selective agar in microbiology. This material…

Q: Which of the following techniques is not used to count bacteria?         Optical density…

A: Bacterial counting can be done by via- (1) Optical density- Optical density of bacteria can be used…

Q: cannot be determined using the details of Gram staining process?   Question 7 options:   bacterial…

A: Gram staining technique is the technique which helps to differentiate between gram positive and gram…

Q: During media preparation, you observed that the agar medium did not solidify after sterilization…

A: Nutrient Agar Medium is the artificial media which is used to grow microbes. It provides essential…

Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…

A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…

Q: Which of the following media selects for gram negative bacteria by inhibiting the growth of gram…

A: Selective media:- it usually allow the growth of a particular microbe and inhibit the growth of…

Q: which bacteria is Coccoid as singles or in pairs, gram positive, non motile and negative oxidase…

A: Answer: BACTERIA = These are the unicellular prokaryotic cells which lacks membrane bound cell…

Q: Which of the following would suggest your stock culture is contaminated? Gram stain indicates…

A: A contaminated culture has had contaminants introduced into it. Contaminants are unwanted microbes…

Q: A laboratory technician performed a series of 10 fold serial  dilutions shown below. he plated 0.1…

A: In this question, we are given colony count from different 10 fold dilutions. The volume plated from…

Q: Which cellular structure is being detected in the motility test? bacterial ribosomes bacterial cilia…

A: Prokaryotes are characterised by the absence of nucleus and membrane bound organelles.

Q: Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium…

A: Bacteria are organisms that are found almost everywhere on earth and are free-living cells that are…

Q: you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each…

A: In the study of microorganisms, a mixed culture is one that contains more than one type of organism…

Q: In the Gram staining procedure, how do the dyes able to distinguish Gram positive from Gram negative…

A: Gram staining, commonly known as Gram's technique, is a staining technique that is utlised to divide…

Q: you are given a mix culture of S aureus, E. coli and P. aeruginosa. besides using the streak plate…

A: The first requirement for physically isolating a bacterium is that it can be cultured in the…

Q: You were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used…

A: "Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: the chemicals used in the gram staining method?

A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…

Q: What technique is used to stain this bacterium? What kind of stain is used? What type of…

A: In this question we have to describe about one of the most important microbiology stain. Please see…

Q: How would you proceed to separate the two bacs in the mixture when you do not have MacConkey and…

A: MacConkey is a special media, different media, indicator media basically use for gram negative…

Q: How can colony morphology be used in the identification of bacterial species? What are the…

A: NOTE: AS PER OUR GUIDELINES, WE ARE ONLY SUPPOSED TO ANSWER THE FIRST THREE QUESTIONS, PLEASE…

Q: Why is low microbial population in milk samples a limitation for microscopic count?

A: The bacteria present in milk are lactococcus , lactobacillus, streptococcus, leuconostoc and…

Q: You place 1 ml of a bacterial broth culture into bottle A containing 99 ml of sterile water and mix.…

A: In serial dilutions, dilutions are multiplied at each step. We can calculate dilution by dividing…

Q: A student is directed to make a simple stain of coli with crystal violet, but got mixed up and used…

A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…

Q: Describe how you would make 10 ml of a 1:500 dilution of bacteria by performing TWO SEPARATE…

A: A serial dilution is the stepwise dilution of a substance in solution. Normally the dilution factor…

Q: Below are morphological, physiological and biochemical characteristics of an unknown luminous…

A: The isolate identified from the above data is Photobacterium.  Photobacterium is a free living, gram…

Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…

A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…

Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?

A: Inoculation is defined as the process of introduction of microorganisms into a culture for their…

Q: bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows:…

A: CFUs are a measure of the number of progenitors present in a given population of cells; if an…

Q: For each of the following stains, list each of the chemicals used (in order), briefly describe what…

A: In Microbiology staining is needed in order to properly visualize the internal membranes of the…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.