I have a mixture of 4 proteins, whose sequences are shown below (note that each protein is a 100-repeat of the sequence shown). Protein A: (Gly-Ala-Lys-Val-Ile-Phe-Glu-Val-Asn-Gly) Protein B: (Ala-Ala-Lys-Arg-Ile-His-Glu-Ala-Asn-Lys) Protein C: (Glu-Val-His-Asp-Ala-Asp-Glu-Val-Asn-Asp) Protein D: (Ala-Lys-Arg-Phe-Trp-Phe-Gly-Ile-Ala-Gly) 1) Which protein/s are MOST LIKELY to precipitate out at a pH of 3.5? a) A b) B c) C d) D e) B & C f) A & D g) None of them h) Cannot be determined.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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I have a mixture of 4 proteins, whose sequences are shown below (note that each protein is a 100-repeat of the sequence shown).

Protein A: (Gly-Ala-Lys-Val-Ile-Phe-Glu-Val-Asn-Gly)

Protein B: (Ala-Ala-Lys-Arg-Ile-His-Glu-Ala-Asn-Lys)

Protein C: (Glu-Val-His-Asp-Ala-Asp-Glu-Val-Asn-Asp)

Protein D: (Ala-Lys-Arg-Phe-Trp-Phe-Gly-Ile-Ala-Gly)

1) Which protein/s are MOST LIKELY to precipitate out at a pH of 3.5?

a) A

b) B

c) C

d) D

e) B & C

f) A & D

g) None of them

h) Cannot be determined. 

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