Identify the applications for gel filtration chromatography. separation of components in a mixture by hydrophobicity estimation of molecular weights separation of components in a mixture by size purification of a specific protein from a mixture

Q: Which of the reactions are spontaneous (favorable)? L-malate + NAD+ oxaloacetate + NADH + H+ AG =…

A: Gibbs free energy change (ΔG) is a thermodynamic quantity that represents the maximum reversible…

Q: 1. Which of the following best describes the relationship between a reactant molecule and an enzyme?…

A: Enzymes are biological catalysts made of proteins. Biochemical processes require enzyme catalyzed…

Q: A protein was purified and then analyzed on a size exclusion column. Its MW was determined to be…

A: SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is used for separating proteins…

Q: Select the choice that best describes the stereochemistry of the following amino acid, and rank the…

A: Cysteine is one of the 20 standard amino acids that serve as the building blocks of proteins. It is…

Q: 12. Receptor immobalization on surfaces for developing bio-sensors

A: Immobilizing receptors on surfaces is essential to developing biosensors, which detect and quantify…

Q: Consider the following peptide: Lys-His-Asp-Glu-Phe-Cys-Ala-Asp-His-Arg What is the net charge of…

A: Amino acids are amphoteric. At a given pH, state of ionization of alpha-carboxylic acid group (pK1),…

Q: Explain why unfolding or aggregation may occur using changes in entropy and enthalpy of the protein…

A: Adding a small amount of salt into a protein solution can increase the solubility of the protein in…

Q: From a kinetics experiment, kcat was determined to be 295sec-1.  For the kinetic assay, 0.3mL of a…

A: Let the initial concentration of the enzyme in the reaction mixture be E0E0 = (Volume of enzyme…

Q: Which of the following define the stereochemistry of alanine (as per the structure shown)? Note:…

A: stereochemistry can be defined as the arrangement of functional groups on the alpha carbon atom with…

Q: Label the structures of the prokaryotic cell. Not all terms will be used. H₂ DESENTAS Answer Bank…

A: Prokaryotic cells can be defined as single-celled organisms. They are known to belong to the Archaea…

Q: Please draw the structure of the 19 L-a-amino acids and proline in any form, as you prefer. You may…

A: Amino acids are the monomer units that make up polypeptides, that eventually folds to become…

Q: Information about poison

A: Poisons are toxic substances or chemicals which interfere the biological process and lead to failing…

Q: 231. Describe how cAMP activity is regulated in the cell.

A: Signal transduction and cellular response depend on cAMP, a secondary messenger molecule. Cells…

Q: Question. How are the relative quantities of these biochemical substances determined?

A: Scientists analyse and quantify biological systems using various experimental methods to determine…

Q: 7. How many liters of a mouthwash can be prepared from 100 mL of cinnamon flavor if its…

A: To prepare a mouthwash with a concentration of 0.5% (v/v) cinnamon flavor, you need to calculate how…

Q: Which of the following statements about enzyme regulation is NOT true? a. covalent modification is…

A: The modulation of enzyme activity in living organisms is referred to as enzyme regulation. As it…

Q: Tyrosine came from the Greek word "tyros" which means cheese as it was discovered in cheese by…

A: Tyrosine is a non-essential amino acid that can be produced in the body by using phenylalanine as a…

Q: DNA sense or template DNA sense or template mRNA tRNA protein 5', 3' amino or carboxyl 5' T U A A RG…

A: The genetic information for protein synthesis is stored in DNA. DNA is double-stranded. The…

Q: pH 1, is the net charge 3 as you said up above or 1 down in the solution

A: When the solution has pH =1 there is no deprotonation occurring because there is no optimum pH…

Q: This image shows the tertiary structure of a protein segment. Tertiary structure results from…

A: Ionic bond is formed between a positively charged and a negatively charged group. The charged groups…

Q: What pairs of sugars represents constitutional isomers, stereoisomers, epimers, enantionmers,…

A: Carbohydrates are polyhydroxy aldehydes or ketones. Monosaccharides are simplest carbohydrates:…

Q: Draw a diagram of a peptide bond in the cis and trans configurations. Show the contributing…

A: The amino group (NH2) of one amino acid and the carboxyl group (COOH) of another amino acid can join…

Q: Do you predict that this salt bridge will become stronger, become weaker, or be unaffected as pH…

A: Salt bridges are attractive interactions between two oppositely charged groups. So, more oppositely…

Q: Name Lysine Arginine Histidine Aspartate Glutamate Structure 1-Let Abbr 3-Let Abbr Class Special…

A: Amino acids are organic compounds that form the building blocks of proteins in cells. The structure…

Q: Draw a non-enzymatic mechanism to describe the formation of the imine between retinal and lysine.…

A: Retinal absorbs a photon and this leads to a conformation change. This leads to the formation of an…

Q: For the equilibrium below, pKa₁ = 7.9 and pKa2 = 10.1. At what pH is [HA] equal to [A²-]?

A: pH of any acid or base tells us its hydrogen ion concentration.PKa- it is that pH when the 50%…

Q: After a polypeptide chain has been synthesized, certain amino acids in the peptide may become…

A: The modified amino acid shown in the structure you provided is serine (Ser).The modification that…

Q: 875.What is the role of a single-stranded DNA binding protein in DNA replication?

A: Formation of replication bubble ( where the two strands of a DNA molecule are separated) is crucial…

Q: You are given a pure protein sample to characterize and provided the following information: Its…

A: 1(A)Beer-Lambert Law is often used in spectrophotometry to quantitatively analyze the concentration…

Q: 1. Analyze the following and identify which one mentions the function of a common eukaryotic ligase?…

A: Enzymes are protein-based biomolecules. They accelerate chemical reactions. They participate in a…

Q: Find the connection among the words below and choose the letter of th word which is different. O A.…

A: DNA fragment separation is accomplished using the agarose gel electrophoresis technique. It…

Q: Q. Define signal transduction and explain the basic components of a signal transduction pathway.

A: Cells perceive external stimuli and respond intracellularly through signal transduction. It's…

Q: 54. Describe the role of an amplifier enzyme.

A: Signal amplification by amplifier enzymes is crucial to many biochemical processes. The initial…

Q: Which one statement below is true about the metabolism of rizatriptan? N N-N ZI rizatriptan MaxaltⓇ…

A: 3.It is a prodrug that is activated by esterases.Rizatriptan is indeed a prodrug, which means it is…

Q: In the disease familial hypercholesterolemia (inherited tendency for high blood LDL cholesterol…

A: Familial hypercholesterolemia (FH) is a hereditary condition characterised by elevated blood levels…

Q: in food microbiology, how do you compute for concentration (M) and absorbance (A) of peroxidase…

A: According to the Beer-Lambert law of spectrophotometry, the amount of light absorbed is directly…

Q: How does the puzzle  look when completed?

A: Any change in the nucleotide sequence of DNA is called mutation. Mutations may be introduced…

Q: DESCRIBE THE MECHANISM FOR FAST LIGAND-GATED CHANNELS AND THE TYPES OF CHANGES THEY CAN PRODUCE IN…

A: Fast ligand-gated ion channels, also known as ionotropic receptors, are a type of cell membrane…

Q: The process by which messenger RNA codes for the synthesis of a specific protein is called: a)…

A: The central dogma of molecular biology is a theory that states that the genetic information encoded…

Q: 22. If 75 mls of 0.02 M HCI is added to 425 mls of 0.5 M glycine buffer at pH 10.4, what is the…

A: Here given that, there is 425 mL of 0.5M glycine buffer at pH 10.4425 mL is equal to 0.425 L…

Q: Supposed you want to use phosphoglucomutase to breakdown glycogen. You found out that this enzyme is…

A: Protein-based biomolecules belong to the class of enzymes. They speed up chemical reactions. Enzymes…

Q: 1.How to prepared SC Yeast Media Buffered with HEPES pH 6.75?

A: In yeast cell culture research, SC (Synthetic Complete) Yeast Media buffered with HEPES at pH 6.75…

Q: Would you expect the pKa to be higher or lower than 8 if the group were buried in the hydrophobic…

A: PKa value is the measure of the tendency of a group to give up a proton. PK a is actually a…

Q: The peptide KNYPEH N-terminal amino group has a pKa of 8.6 and the C-terminal carboxylate group has…

A: At pH 1, both the N-terminal amino group and the C-terminal carboxylate group will be protonated.The…

Q: 1. In a catabolic pathway, metabolite X gets converted into metabolite Y, metabolite X metabolite…

A: is the standard change in Gibbs free energy i.e. the change in Gibbs free energy under standard…

Q: Which solution conditions would be best used for eluting a transcription factor from a DNA affinity…

A: We need to identify the solution conditions used for eluting a transcription factor from a DNA…

Q: The compound below, doxorubicin, is a substrate for p-glycoprotein. Cancer cells with a higher…

A: Cancer is mainly caused by the uncontrolled proliferation of cells due to mutation. Doxorubicin is…

Q: Identify which structures are equivalent to the L-configuration. ☐ CH3 H- H₂N- -NH₂ COOH CH3 -H COOH…

A: In biochemistry, amino acids are the building blocks of proteins, and they come in two different…

Q: - Multiple choice Allopurinol is an inhibitor of xanthine oxidase. Administration of allopurinol to…

A: Xanthine oxidase is inhibited by allopurinol. This enzyme is involved in the conversion of xanthine…

Q: 8. When oxygen binds to the heme group, by how many angstroms does the Fe2+ appear to shift? 9. How…

A: The heme group is a prosthetic group in oxygen-binding protein. Its primary function is to bind…