If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many CFUs per mL were in your original sample? If a bacterium can divide once an hour, then after 5 hours, 1 bacterium will have yielded ______ bacteria
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If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many CFUs per mL were in your original sample?
If a bacterium can divide once an hour, then after 5 hours, 1 bacterium will have yielded ______ bacteria
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- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).If 1.0 mL of a 1:1000 dilution produces 200 colonies on a culture plate, how many bacteria were present per mL in the original (undiluted) sample? 200,000 20,000 2,000 200To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a final volume of 5 ml. How did you calculate it?. Single line text.
- a pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volumeA sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.
- A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40 bacteria what would be the dilution scheme of using 4 dilutionsGiven: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.
- You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into blanks A, B, and C, respectively). Following spread plating and incubation of a 0.1 mL subsample from Blank C, you count 66 colonies on the plate. How many colony-forming units are in the original broth culture?John performed a side-by-side Gram satin of a known Gram positive and known Gram negative organism. Unfortunately, he forgot to do iodine step. When he observed his completed slide under oil immersion, what do you think he saw?