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- How would you draw out a step in an experiment that included the following? Please help, I am so lost. Metal coupon inoculation (vortex/bead method) and assessment of viable cells by culture to simulate wet fomite or dry touch surface contamination.procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slanta pure bacterial culture was diluted by adding a 0.2 mL aliquot to 0.9mL water. Then 0.1 mL of this dilution was plated out, yielding 82 colonies. Calculate the CFU/mL in the original culture.
- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (1) broth culture to brothA bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?EXPERIMENT : CELL COUNTING FOR DETERMINING VIABLE CELL CONCENTRATION Objectives: To count adherent and suspension cell using hemocytometer. To calculate viable cell concentration in a suspension. Procedures: Counting Viable Cells using Hemocytometer/Automated Cell Counter▪ Prepare the hemocytometer slide by cleaning the surface with 70% ethanol carefully (do not scratch the semi-silvered coating). Apply the same step for the coverslip. Wet the edges very lightly and press it down over the grooves and semi- silvered counting area. ▪ Collect 20 μl samples from the cell suspension using a pipettor and transfer it immediately to the edge of the hemocytometer chamber. ▪ Expel the suspension and allow it to be drawn under the coverslip by capillary force. Do not overfill the chamber. Blot off any surplus liquid and place the slide under the microscope. ▪ Alternatively, trypan blue dye can be used to stain cells by mixing an equal volume of trypan blue to a cell suspension (1:1) to…
- If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original cultureYou aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is the dilution factor. Show workDilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you add to the new media?
- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features observable under the high-power objective and their movement as seen in the hanging drop preparationIf you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many CFUs per mL were in your original sample? If a bacterium can divide once an hour, then after 5 hours, 1 bacterium will have yielded ______ bacteria