Set of chromosomes Add PCR primers. The PCR primers specifically recognize sequences on chromosome 2 that flank a particular microsatellite. Microsatellite- Microsatellite Many cycles of PCR produce a large amount of the DNA fragment contained between the 2 primers. The DNA is subjected to gel electrophoresis. Microsatellite with more repeats - Microsatellite with fewer repeats FIGURE 23.5 Identifying a microsatellite using PCR primers.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter17: Metabolism: An Overview
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Problem 13P: What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome...
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What causes microsatellites to be polymorphic?

Set of
chromosomes
Add PCR
primers.
The PCR
primers specifically
recognize sequences
on chromosome 2
that flank a particular
microsatellite.
Transcribed Image Text:Set of chromosomes Add PCR primers. The PCR primers specifically recognize sequences on chromosome 2 that flank a particular microsatellite.
Microsatellite-
Microsatellite
Many cycles of PCR
produce a large amount
of the DNA fragment
contained between
the 2 primers. The
DNA is subjected to
gel electrophoresis.
Microsatellite
with more repeats
- Microsatellite
with fewer repeats
FIGURE 23.5 Identifying a microsatellite using PCR primers.
Transcribed Image Text:Microsatellite- Microsatellite Many cycles of PCR produce a large amount of the DNA fragment contained between the 2 primers. The DNA is subjected to gel electrophoresis. Microsatellite with more repeats - Microsatellite with fewer repeats FIGURE 23.5 Identifying a microsatellite using PCR primers.
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