In the isolation of protein casein from milk, why is heated distilled water added into the milk, what is the purpose of warm distilled water?
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In the isolation of protein casein from milk, why is heated distilled water added into the milk, what is the purpose of warm distilled water?
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- Are all the proteins separated properly using SDS-PAGE? Why or why not?When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reason for such an observation?Why was an SDS-PAGE gel used when working with a protein as opposed to an agarose gel? What dye was used for this gel?
- an protein has a molecular wieght of 90 KD when purified by gel filtration and has a molecular wieght of 60 KD hen purified by electrophoresis which one is the more selective?If 1ml of an unknown sample of protein is diluted to 10ml, and a 0.5ml portion of the diluted sample is found to contain 50ug of protein when assayed for protein using the lowry method. What is the concentration of protein in ug/ml in the original sample?In an experiment of egg white and calamansi juice in protein denaturation, what will happen?
- Give an example of a scenario in which you could partially isolate a protein with differential centrifugation using only one spin.I hope you can help me with this. When developing the zymogram using a destaining solution, a researcher observed wedge shaped bands with protein. What could be the possible reasons for such an observation?For the following sequence, what is the approximate annealing temperature? 5'-AGCTACGATCAGGTCA-3'
- What is the principle behind the very specific sequence in donning and doffing PPEs? ExplainIf 1ml of an unknown sample of protein is diluted to 10ml, and a 0.5ml portion of the diluted sample is found to contain 50ug of protein when assayed for protein using the lowry method. If you mix two unknown samples and repeat the lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used?The oligonucleotide d-ATGCCTGACT was subjected to sequencing by Sanger’s dideoxy method, and the products were analyzed by electrophoresis on a polyacrylamide. Draw a diagram of the gel banding pattern obtained.