Q: What is the source of Kinins?
A: Kinins are small peptides. It is broken down by kininases. They act on phospholipase and increase…
Q: is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a…
A: Protein concentration determination is Pa quantitative estimation that can be done in many ways and…
Q: Explain the biochemical functions and deficiency manifestation ofVit D ?
A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…
Q: technique is used to identify the binding partner for Izumo?
A: Sexual reproduction helps in the increase in progeny of almost all eukaryotic organisms. Sperm meets…
Q: Are all the proteins separated properly using SDS-PAGE? Why or why not?
A: Proteins are massive biomolecules and macromolecules made up of one or more long chains of amino…
Q: Explain the synthesis process of Losartan? Please answer at your own words
A: The process in the synthesis of losartan are: Alkylation of methanol…
Q: What is the function of conjugation reactions in the biotransformation process?
A: Biotransformation refers to reactions that modify a specific chemical compound (both endogenous and…
Q: One of the blocking solutions that is traditionally used is non-fat milk, but this is not…
A: A phosphoprotein is a protein that has a single phosphate group attached to it, or a complex…
Q: What is neomycin phosphotransferase (designated neor)?
A: Antibiotics are prescriptions that battle bacterial diseases in individuals and creatures. They work…
Q: I did an SDS PAGE with BSA as a standard; I noticed in my results that there is a double band - is…
A: SDS-PAGE is Sodium Deodecyl Sulphate Polyacrylamide Gel Electrophoresis. It is a technique that is…
Q: Explain the DNA fragments of agarose gel ?
A: Agarose is extracted from the red seaweed and basically, it is a polysaccharide. The main use of…
Q: What is Folate Trap ? Explain the importance of Folate Trap ?
A: In a human body due to deficiency of vitamin B12 folic acid is rendered inactive this phenomenon is…
Q: Can someone give me examples of denatured proteins that can be brought back to native state…
A: The question asks the examples of denatured protein which can be transformed into the renatured or…
Q: Why is RNA yeast soluble in diluted NaOH solution?
A: Ribonucleic acid (RNA) is an important biological macromolecule that is present in all biological…
Q: What are the necessary conditions for the generation okazaki fragments?
A: The short sequences of DNA which are formed by at the lagging strand during replication is called…
Q: is Polymyxin a cationic antimcrobial peptide? Does Aureomycin targets lipopolysaccharide?
A: is Polymyxin a cationic antimcrobial peptide? Yes
Q: What is the primary function of chaperones? How docells sometimes reuse partially denatured…
A: In accordance with the molecular biology, the molecular chaperones are the proteins that help in the…
Q: How is ATCase regulated to generate precisely the amount of pyrimidines needed by the cell?
A: Aspartate transcarbamoylase (ATCase) catalyzes the first step of pyrimidine synthesis. It converts…
Q: Based on your Lineweaver-Burk plot, what is the mode of PaESBL-1 inhibition by NSAMB?
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Describe the stucture of triacylglycerols (TGA) and explain the hydrolysis of TAGs
A: Carboxylic acid with hydrocarbon side chain are fatty acids. Triacylglycerol are formed from…
Q: What is Fluorescence? And how does GFP exhibit fluorescent property?
A: Fluorescence is a type of luminescence that occurs in gas, liquid, or solid chemical systems. Green…
Q: What defect in the cell causes the condition known as Zellweger's syndrome, ZS? Explain in detail…
A: Zellweger syndrome is the most severe form of the Zellweger spectrum of conditions. It is a rare…
Q: Define the term thiolytic cleavage. In what biochemical process does it occur?
A: A reaction in which a bond to a sulfur atom is broken is known as the thiolytic cleavage. In the…
Q: How can homo- and heterofermentative metabolism bedifferentiated in pure cultures of lactic acid…
A: The process of Bacterial contamination during industrial yeast fermentation has severe economic…
Q: Why is ti advantageous for a cell to expend metabolic energy to polymerize gulucose molecule.?
A: Human blood possesses sugar in the form of glucose, it is being carried to all cells as a source of…
Q: Under what circumstances should the product Ocuser-pilo be used? Compare the advantages and…
A: The devices that are sterile, thin, multilayered, drug-impregnated and administered into the…
Q: Consider the leucine transporter. Would Vmax and/or Kt change if you added a Na+ ionophore to the…
A: As glucose is pumped from the intestine into the epithelial cell at the apical surface, it is…
Q: What is the name of themolecule that transportsoxygen in red blood cells?
A: The red blood cells (RBC) are enucleated cells that transport oxygen molecules from the lungs to…
Q: What is isosterism and bioisostreism? How does this concept help in biotransformation of different…
A: In biology, drug designing is a major aspect. Drug specificity and effectivity are determined by…
Q: If the molecule can inhibit toxic effects at 0.5mM against gastric carcinoma cells, does it mean…
A: IC50 is the half inhibitory maximum concentration of a drug which inhibits the process 50%.
Q: In the isolation of protein casein from milk, why is heated distilled water added into the milk,…
A: Casein is an abundant protein of milk which is globular in structure.It has iso electric point of…
Q: What fragments can be obtained from the treatment of Pro-Lys-Gly-Gly-Arg-Trp-His-Cys with trypsin…
A: The digestive enzymes trypsin and chymotrypsin are secreted by the pancreas as inactive enzyme…
Q: How does cleavage of a single peptide bond activate the zymogen?
A: Zymogen, is called proenzyme, with no catalytic activity. However, they are transformed into…
Q: Why is hudroxychloroquine or chloroquine detrimental against COVID-19?
A: The coronavirus outbreak, which emerged in December 2019 in Wuhan, China, has developed into a…
Q: COVID-19
A: Respiratory system is a biological system which consists of network of organs and tissues that help…
Q: What component of the extracellular environment is required for the homophilic interactions mediated…
A: Cadherin is a type of adhesion molecule that exists within cells. Cadherin is found almost…
Q: Is there a way to classify or identify the tests needed in order to differentiate C12H22O11 and…
A: C12H22O11 is known as sucrose. It is a disaccharide sugar, formed by glucose and fructose. It is…
Q: Give an example of a scenario in which you could partially isolate a protein with differential…
A: To find out the amount of ammonium sulfate to the added to a protein solution to precipitate the…
Q: How liquid chromatography is used in protein purification?
A: Proteins, one of the major categories of macromolecules present in living organisms, are responsible…
Q: What is quorum sensing?
A: It was discovered by Kenneth Nealson, Tery Platt, and J.W. Hastings in the year 1970. Many living…
Q: describe how the fluorescence spectrum is changed when acid and BME are added to BSA and lysozyme,…
A: In biochemistry, fluorescence spectroscopy is defined as the electromagnetic spectrum that…
Q: What is a protease enzyme? Why would we treat the strawberry extract with a protease in an…
A: DNA isolation or DNA extraction is the method used to isolate DNA from a particular sample which is…
Q: What is cisacting site ?
A: cis means " same side". The cis acting site acts as a site of DNA or RNA which helps in regulating…
Q: What is the expansion of acronym MICA?
A: Immune system includes cells and molecules involved in protecting body against antigens.
Q: what are serine protease ? give examples.
A: Protease , also known as peptidase or proteinases are the enzymes that catalyses proteolysis ,…
Q: Is there a difference in the effects of different salt solutions on protein denaturation? Why?
A: Yes, there is a difference in the effects of different salt solutions on protein denaturation.…
Q: What is the structure of ATCase?
A: Aspartate Transcarbamoylase (ATCase) catalyses the initial step in the biosynthesis of pyrimidines…
Q: What are statins? Name the microorganism that produces this substance. How is it medically…
A: A drug is a molecule which might resemble a organic molecule inside the body or an exogenous…
Q: Which biochemical processes might be affected by a deficiency of thiamine?
A: The normal function of the human body relies on proper nutrition which can be derived from vitamins…
How might the technique of affinity chromatography (see animation) be used to purify lectins?
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- Why are integral proteins more difficult to study then peripheral, and why integral can only be isolated with strong deteargents.Descripe A-B-C-D regions on the agarose gel , what does these regions mean ?What is the primary function of chaperones? How docells sometimes reuse partially denatured proteins?
- What are the components of the buffers used for protein hydrophobic column chromatography and explain how do they work?Why is a strong reducing agent, such as NaBH4, not suitable for use in the conjugation of an oligosaccharide to a protein?What protein purification methods can be used to ensure that the resulting protein is soluble ?
- Find a method that uses some form of HPLC for the analysis of proteins. What was the stationary phase used? How does this kind of stationary phase separate the proteins? What kind of mobile phase was used? Was the method isocratic or was a gradient used? How were the proteins detected?Upon doing the experiment of Protein Denaturation, what could be observed in the precipitation of heavy metal ions if an albumin solution in a solution is dropped with drops of silver nitrate and then it will be shaken? Explain.How liquid chromatography is used in protein purification?