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Interpret The IR graph identifying peaks for Nitration
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- The students conducted the assay for LDH activity using the two serum samples. Each cuvette (path length 1 cm) contained 3ml of a suitable assay buffer (including pyruvate as substrate). 20 microlitres of either serum was added to the cuvette and the absorbance values immediately recorded at the optimum wavelength for a period of 5 minutes (absorbance readings taken every 30 seconds). Protein concentration of serum sample (mg/ml) Change in absorbance at optimum wavelength per minute Control serum (C) 8 -0.04 Diseased serum (D) 7.8 -0.6 1c. Using the molar absorption coefficient of NADH as 6220 M-1 cm-1, and by application of the Beer-Lambert law, estimate the enzyme activity in the two samples (C and D). Express activity as moles per second. 1d. Estimate the specific activity of the two samples (moles per second per microgram).chemistry help with correct sig -figFT-IR technique can be utilized for the analysis of unknown analytes by matching it with__________. Both choices are correct Commercial library/database Reference standard
- Using the raw data, pls fill the table accordingly. I will thumbs up if done properly.List three typical examples applications of SFC analysis.Initial temp: 19.06 C trial 1: 0.5 M concentration trial 2 : 1.0 M concentration 200 mL of HCl and 200 mL of NaOH are combined in an insulated container. HCl = 0.5 mol/ml x 200 x 10^-3 ml = 0.1 mol NaOH = 0.5 mol/ml x 200 x 10^-3 ml= 0.1 mol H+(aq) + OH-(aq) - - -> H2O (l) ∆H H++ = 0, ∆H OH−− = -230 kJ/mol, ∆H H2O = -286 kJ/mol ∆H reactants = ∆H H++ + ∆H OH−− = -230 + 0 = -230 kJ/mol ∆H reactants = -286 kJ/mol - ( -230 kJ/mol) = -56 kJ/mol For each trial, calculate how much energy is released during this reaction.
- pls help very very urgent, explanation is not requiredAnionic surfactants in wastewater are measured spectrophotometrically as methylene blue active substances (MBAS). A volume of a water sample is first reacted with methylene blue, a cationic dye, in a basic buffered aqueous solution. The resulting solution is agitated after the addition of chloroform. The organic layer is then extracted, and the absorbance is read at 655 nm. A standard calibration curve is produced by plotting absorbance vs. concentration for the concentration range between 0 ppm to 2.50 ppm. The line had the following equation: y = 0.286x + 0.009 a. The limit of surfactant content in Philippine class B water is 300 ppb. If a 5 mL aliquot of a 50 mL water sample had an absorbance of 0.084 after MBAS analysis, is the sampled water body suitable for bathing? Support your answerA 0.0200 gram blood sample was decomposed by a microwave digestion technique followed by dilution to 100.0 mL in a volumetric flask. Aliquots of the sample solution were treated with a lead complexing reagent and water as follows: Solution 1: 10.0 ml blood sample + 20.0 mL complexing agent + 30.0 mL H20. Solution 2: 10.0 ml blood sample + 20.0 mL complexing agent + 26.0 mL H20 + 4.00 mL of 78 ppb Pb2+ standard. The resulting solutions were analyzed by UV/Vis at 375 nm. Absorbance for solution 1 = 0.155 and for solution 2 = 0.216. Calculate the concentration of lead (ppb) in the original sample.
- If you chose 6 reading for Water-Cut Analyzers (BS&W) macheen, Answer the qustions: Day1 Day2 Day3 Day 4 Day 5 Day 6 0.05 0.06 0.07 0.05 0.04 0.05 - Evaluate the accuracy and precision of the selected data. - Estimate the sources of errors in selected data (systematic & random error). - Explain the effect of these errors in the process.Chemistry Column 25 m length and 0.2 mm I.D. has efficiency is 100,000 theoretical plates; Flow rate is 1 mL/min. Calculate the peak width for component with retention factor 2. Which data acquisition sampling rate should be used for proper quantification of this component? Explain. (For accurate integration peak should have at leastExplain why internal standard can be used for ICP-OES but is not used for AAS