Lead Acetate Reaction To 1 mL of the sample in a test tube add 1 mL of 40% NaOH. Boil the content for at least 3 minutes. After cooling, add 2 – 3 drops of 10% Pb(OAc)2. Note the color of the solution or precipitate formed. Question 1. What happened to the proteins after adding 40% NaOH and heating the solution? Question 2. What specific amino acid is detected in this reaction? Question 3. What is the identity of the precipitate, if there is any?
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Lead Acetate Reaction
To 1 mL of the sample in a test tube add 1 mL of 40% NaOH. Boil the content for at least 3 minutes. After cooling, add 2 – 3 drops of 10% Pb(OAc)2. Note the color of the solution or precipitate formed.
Question 1. What happened to the proteins after adding 40% NaOH and heating the solution?
Question 2. What specific amino acid is detected in this reaction?
Question 3. What is the identity of the precipitate, if there is any?
Step by step
Solved in 2 steps
- Millon-Nasse Test Mix 1 mL of the sample and 5 drops of Millon-Nasse reagent. Boil the content gently for 30 seconds. Cool in water. Add 2 drops of freshly prepared 1% NaNO2 and warm gently. Observe the color of the precipitate and/or the solution. Question 1. Will all amino acids and proteins give a positive reaction to this test?Question 2. What causes the color of the solution or precipitate?What test reagent is most sensitive in detecting sugar in urine? What are the possible positive results (color) and its interpretation? What test could be used to differentiate: sucrose and lactose? Explain. glucose and starch? Explain. glucose and fructose? Explain. galactose and fructose? Explain An unknown carbohydrate gave a brick red precipitate when tested with Fehling's reagent, turned red when reacted with Seliwanoff's reagent, and quickly gave a red precipitate when reacted with Barfoed's reagent. What conclusions can be made about this carbohydrate? Illustrate with equation the reaction of glucose, galactose, maltose & lactose with osazone test. Sketch the crystals formed for each of the following sugars examined under the microscope.A. Biuret TestMix 1 mL of sample with 1 mL of 5% NaOH. Add 2 – 3 drops of 1% CuSO4. Question 1: What is the chemical equation of the reaction. Will it give positive reactions to all types of proteins? How about peptides and amino acids? Explain briefly.
- Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in this experiment. For each test, cite the respective application and describe the positive result. test that have been performed a.Ninhydrin Test b.Biuret’s Test c.Xanthoproteic Test d.Millon’s Test e.Sakaguchi test f.Hopkins –Cole Test g.Sulfur Reaction or Lead Acetate TestThe hydrolysis of starch was stopped when the iodine test no longer gave a blue color. Does this mean that the starch was completely hydrolyzed? Explain.In the acid- catalyzed hydrolysis of starch, how can you tell when the hydrolysis of starch is complete, why does the test work this way? What is the monosaccharide at the end?
- In what way can alkaloidal reagents precipitate proteins? In excess of alkaloidal reagent, did the precipitate formed dissolve or not? What is the evidence for your answer?Inulin is a polysaccharide compound entirely of fructose units. What would be the effect of the seliwanoff's test on inulin? Explain in 2-3 sentences.Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
- 1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.MATCHING TYPE; Match A to B A. Acrolein tesT Benedicts test Biuret test Iodine test Barfoed’s test Molisch test Seliwanoff’s test Fehling’s test Salkowski’s test Furter-Meyer test B. A. The test for the presence of cholesterol. B. The test for reducing sugars in acidic solution. C. The test is used to detect cholesterol in a solution D. The test for the presence of starch/amylose. E. The test for presence of reducing sugars in alkali solution. F. The general test for carbohydrates. G. This method is used to detect the presence of tocopherols H. The test to distinguish monosaccharides from disaccharides I. The test for the presence of fats or glycerin. J. The test to determine ketohexose from an aldohexose.What would be the biuret test result if the specimens tested are alanine and casein? What group in amino acid is responsible for a positive ninhydrin test? What would be the Ninhydrin test result for Glycine and Aspartic acid? What amino acid is present in proteins is responsible for a positive Millon’s test?