VGIYLQKTSDHRPEFALAMN What is its isoelectric point of this peptide?
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VGIYLQKTSDHRPEFALAMN
What is its isoelectric point of this peptide?
Isoelectric point is the pH at which the net charge is zero. pI is calculated as the average of two nearby pka values. Proteins are polymer of amino acids linked by peptide bond/amide bond (covalent) with release of a water molecule. Alpha carbon of amino acids contains -carboxyl group, amine group, side chain group and hydrogen atom. Based on the side chain group amino acids can be classified as polar, uncharged polar and non-polar.
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- THOUGHT QUESTION Imagine we identify a gene that is directly responsible for the effects of vasopressin on male mammals, including humans-we will call it trust1-that leads to the production of a vasopressin receptor in the brain, which we will call TRUST1. There are different versions of trust1, all of which lead to different levels of the behavior associated with this neuropeptide on male behavior. Give some examples where it would be a good idea to know a particular males genotype-that is, which of the trust1 genes he has. Give an example of when you think science has gone too far and this information should not be known.1CFD CALCIUM-FREE CALMODULIN How long is the protein’s primary sequence?Does this protein have any secondary, tertiary and/or quaternary structures present?Are there any molecular additions to this protein that are not amino acWhich peptide would absorb the most UV light at 280nm? (Can you show work because I mainly want to know how to solve this type of problem. Thank you!)Leu-Trp-Tyr-Ala Tyr-Lys-Tyr-Cys Glu-Tyr-Ile-Arg Ala-Trp-Trp-Ala Thr-Ala-Ile-Thr
- If you were to remove the ER retrieval signal fromprotein disulfide isomerase (PDI), which is normally a sol-uble resident of the ER lumen, where would you expect themodified PDI to be located?Vasopressin: CYFQNCPRG Oxytocin: CYIQNCPLG Both of these peptides will react with reducing agents such as beta mercaptoethnol (BME). What side group reacts with this reducing agent? What does the fact that these peptides both react with BME tell you about the side groups and therefore this peptide?Following the IUPAC rules for naming small peptides, assign a name for Leu–Lys–Trp–Met
