Modified True or False: Write TRUE if the statement is correct, if the statement is false, change the word/ words in (parenthesis) to make it correct 1. In (ribonucleotides), the pentose sugar is deoxyribose 2. (Helicase) is an enzymes that untwist the double helix at the replication forks, separating the two parental strands and making them available as template strands. 3. (DNA polymerase I) bind to the unpaired DNA strands, keeping them from re-pairing.

Modified True or False: Write TRUE if the statement is correct, if the statement is false, change the word/ words in (parenthesis) to make it correct 1. In (ribonucleotides), the pentose sugar is deoxyribose 2. (Helicase) is an enzymes that untwist the double helix at the replication forks, separating the two parental strands and making them available as template strands. 3. (DNA polymerase I) bind to the unpaired DNA strands, keeping them from re-pairing.

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 17P

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MULTIPLE CHOICE 1. What enzyme that function for cutting a DNA? A. Nuclease B. Ligase C. Primase D. Isomerase 2. Which of the following does not involved in the proofreading and repair of DNA? A. Isomerase B. Ligase C. DNA Polymerase I D. Nuclease
True or False: 1. There is a reduction in the length of the DNA after every round of replication. 2. When transcribing, growing transcripts always run towards the 5’end of the template strand.
True or false? DNA repair mechanisms all depend on the existence of two copies of the genetic information, one in each of the homologous chromosomes. DNA primase makes DNA primers for the synthesis of Okazaki fragments in the DNA lagging strand. The repair of double-stranded breaks by nonhomologous end joining is accurate. RNA polymerase III transcribes all protein-coding genes.
During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase
5'-[seq]-3' The diagram shows the results of gel electrophoresis for Sanger sequencing. The wells are represented by open boxes and the DNA bands are represented by black boxes. The wells are labeled to show which dideoxy reaction was loaded into each. Write the sequence of the original template strand used for this sequencing reaction, with the 5’ end on the left and the 3’ end on the right.
INSTRUCTION: Given the DNA sequence below, provide the answers to the following items. a. complimentary DNA strand 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C b. mRNA 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C c. protein synthesized 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C
Please help me to answer the following: 1. Explain how the synthesis of a DNA daughter strand growing toward a replication fork differs from the synthesis of a daughter strand growing away from a replication fork. 2. The base sequence ACGTCT represents a portion of a single strand of DNA. Please draw the complete double stranded molecule for this portion of the strand and use the representation to illustrate the replication of the DNA strand.Please clearly identify the nucleotide bases involved, the new strands formed, and the daughter DNA molecules. 3. Please explain the origin of Okazaki fragments. Thank you very muxh for your help.
Please answer all correctly. Thanks. 1. When DNA helicase is active, the result is: - annealing of RNA primers to the DNA - formation of phosphodiester bonds - formation of hydrogen bonds - separation of the two strands of the double helix - swivelling of the single separated strands of DNA 2. During DNA replication _______ aids in relieving pressure from DNA supercoiling. - helicase - RNA polymerase - ligase - topoisomerase
Multiple Choice 1. How does frameshift mutations can result from? A. the substitution of a stop codon for an amino acid-specifying codon. B. the substitution of a start codon for an amino acid codon. C. the insertion or deletion of one to three base pairs. D. the substitution of one base pair for another. 2. Which of the following statement is considered false during DNA replication? A. The replication process undergone in the cytoplasm of prokaryotes. B. The replication process goes on 3’ ->5’ end. C. The replication process goes on 5’ ->3’ end. D. The replication process undergone in the nucleus of eukaryotes.
1d) Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?
True or False? 454 platform sequencing involves attaching single -stranded DNA fragments to beads, which are embedded within a 96-well plate . The plate is exposed to separate washes of single nucleotides , which are used by DNA polymerase to synthesize complementary DNA. Each time a nucleotide is added, a pyrophosphate is released.
Multiple Choice 1. Which of the following statement is considered false during DNA replication? A. The replication process undergone in the cytoplasm of prokaryotes. B. The replication process goes on 3’ ->5’ end. C. The replication process goes on 5’ ->3’ end. D. The replication process undergone in the nucleus of eukaryotes. 2. Which of the following statements best describes the relationship between the nucleotide sequences of the template strand and the non-template strand in the DNA of a gene? A. They are parallel to each other. B. They are complementary to each other. C. They are identical to each other. D. They are covalently attached to each other.