Sliding clamp- Clamp loader DNA polymerase- 44| |45 dCMP hydroxymethylase- 62 43 42 40 Helicase-41 Primase-61- dCTPase-dUTPase-56- 60 20 Helicase-dda. 1-DNMP kinase 39 60- Topoisomerase O Reference point 160 80- 52 100 Single-stranded 32 DNA binding protein 140 120 DNA ligase – 30 FIGURE 22.11 Partial map of the T4 genome. Genes whose products participate in DNA metabolism are shown on the outside of the circular map. The inner numbers represent distances in kbp from the reference point (0), which represents the divide between two genes rlIA and rlIB (not shown).
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
Bacteriophage T4 has a linear double-stranded DNA genome, yet mapping many mutations, as shown in Figure, generates a circular linkage map. How might you explain this discrepancy?
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