Ninhydrin Test Reagent’s Composition Observations: Inferences: What is the principle behind this test? Can ninhydrin test distinguish amino acids and proteins? Can this test be used to detect the presence of proteins in a sample? Elaborate your answers.
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Ninhydrin Test
| Reagent’s Composition | |
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Observations: |
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Inferences: |
What is the principle behind this test?
Can ninhydrin test distinguish amino acids and proteins? Can this test be used to detect the presence of proteins in a sample? Elaborate your answers.
|
Step by step
Solved in 2 steps
- Ninhydrin test for amino acids or protein requires that sample test solution should be at initially basic pH. True or False?Based on the following qualitative tests for proteins, which one is positive/negative for intact protein, acid hydrolysate, and base hydrolysate? Biuret Test Ninhydrin test Sakaguchi test Xanthoprotein test Hopkins-Cole test Lead acetate test Millon’s testDiscuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and saponification to differentiate TAGs, phospholipid, and cholesterol. What are the general reactions involved? Include chemical reactions if necessary.
- Which organic molecules may be found in urine from a patient with kidney illness, and which biochemical test would be the most suited for detecting those molecues?In protein electrophoresis, what reagent present in the sample buffer is used to eliminate differences in the charge densities of proteins? Tris-HCL ph 8.8 2-mecraptothanol SDS(sodium dodecyl sulfate) Tris-HCL PH 6.8Which of the following correctly matches the chromatography technique with the molecular property being exploited for separation? ion-exchange: charge,affinity: polarity, gel-filtration: molecular mass ion-exchange: charge, affinity:molecular binding preference,gel-filtration: molecular size ion-exchange: charge,affinity: molecular binding preference,gel-filtration: isoilectric point ion-exchange: ion binding preference,affinity: molecular size,gel-filtration:polarity
- Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073xMultiple choice Which compound will yield a positive result with Salkowski test? (Picture 1) A, B, C, D In the emulsification of fats, which species is water soluble? (Picture 2) A, B, C, D In the emulsification of fats, which species is hydrophobic? (Picture 2) A, B, C, DThe following were obtained when testing for lipids. Discuss and conclude from the results obtained.
- When comparing the biuret reaction to measuring protein absorbance directly at 280 nm, list two benefits of utilizing the biuret reaction to quantify protein concentration.Describe the principles involved in the separation of proteins by sodium dodecyle sulphate (SDS) PAGE and isoelectric focusing.You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.