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- A Deficiency on 3-Phosphogtycerate Dehydrogenase Can Affect Amino Acid Metabolism Although serine is a nonessential amino acid, serine deficiency syndrome has been observed in humans. One such form of the syndrome is traceable to a deficiency in 3-phosphoglycerate dehydrogenase (see Figure 25.31). Individuals with this syndrome not only arc serine-deficient but also arc impaired in their ability to synthesize another common ammo acid, as well as a class of lipids. Describe why.Understanding the Mechanisms of Reactions Related to Transketolase The mechanistic chemistry of the acetolactate synthase and phosphoketolase reactions (shown here) is similar to that of the transketolase reaction (Figure 22.30). Write suitable mechanisms for these reactions.The first step of gluconeogenesis involves the carboxylation of pyruvate and has a large negative delta G (i.e very exergonic). Explain how it is possible that this step can be exergonic if we know that adding CO2 onto molecules is fundamentally a destabilizing phenomenon?
- Pyruvate dehydrogenase catalyzes the conversion of pyruvate to acetyl CoA. In this reaction, one molecule each of the catalytic coenzymes TPP, FAD and lipoamide participate in the reaction but are not consumed. OTrue O FalseThe activity of the enzyme β-galactosidase produced bywild-type cells grown in media supplemented with different carbon sources is measured. In relative units, thefollowing levels of activity are found:Glucose Lactose Lactose + glucose0 100 1Predict the relative levels of β-galactosidase activity incells grown under similar conditions when the cells arelacI−, lacIS, lacO+, and crp−.Malonyl-CoA synthesized by the action of acetyl-CoA carboxylase II is primarily used:a. To synthesize fatty acidsb. To inhibit fatty acid oxidationc. Both a and bd. Neither a nor b
- Upon considering the complete oxidation of one mole of fatty acyl (CoA) of an arachidic acid (20:0). Answer the following lettersa. total no. of NADH produced from the oxidation of all acetyl CoA in the acetyl cycleb. otal no. of FADH2 produced from the oxidation of all acetyl CoA in the acetyl cyclec. total atp yieldATP is a (+) allosteric effector, and CTP is a (-) allosteric effector of theenzyme ATCase. Both of these heterotropic effectors bind to the regulatorysubunits on ATCase. The substrates of ATCase, aspartate and carbamoylphosphate, bind the enzyme active site with positive cooperativity (i.e.,they exert a “+” homotropic effect on activity). As the concentrations ofthe substrates change from values where [S] ≪ KM to values where [S] issaturating ([S]≫ KM), how will the binding constants for each of the twoallosteric effectors change? In other words, does ATP bind ATCase withhigher affinity when [S] is low or high? Does CTP bind ATCase with higheraffinity when [S] is low or high?Give answer of 1,2,3, MCQ's 1. Which of the following statements about delto-ALA (amino amino ievulinate Synthases are FALSE? A It catalyzes the rate limiting step in the pathway of heme synthesis B. It is allosterically activated by heme C. It synthesizes delta-ALA from glycine and acetyl-CoA D. It has a decreased activity in case of vitamin 87 deficiency E. Heme influences the concentration of delta-ALA synthase in the red blood cell precursor. 2. Select the correct statements about heme degradation: A. It involves the removal of Fe2+ from heme molecule with the generation of protoporphyrin B. During heme degradation, the protoporphyrin ring is opened and CO is released C Bilirubin is conjugated with UDP glucuronic in the spleen D. The conjugated bilirubin is transported in plasma by albumin E. The stercobilin that arises by the oxidation of urobilinogen confers the characteristic color of the feces 3. Which of the following represent possible consequences of an intense hemolysis…
- Which of the following coenzymes does not get restored to it's original form within the PDH complex once pyruvate gets converted to acetyl CoA a. NAD+ b. TPP c. Lipolic Acid d. FADThere are two steps in the TCA cycle that involve the release of free CoA-SH. However, only one of these steps is regulated (i.e. only one of the steps has a large negative delta G). Explain why one of these CoA-SH releasing steps is highly thermodynamically favorable while the other is not.Upon the addition of diazonium salts, ketoacids like glutamate and pyruvate forms in the presence of hydroxide as a color developer in Reitman Frankel Method. At pH 6.8, Pyruvate kinase catalyzes the transfer of phosphate from phosphoenolpyruvate to ADP to become ATP a. both false b. both are true c.. only second is true d. only first is true 2. constriction of the sphincter of oddi may cause falsely ______ in ____ activity a. none of the choices b. decresed, lipase c. increased, ck-mb d. increaased, amylase e. decreased, LDH 3. The amyloclastic method of amylase activity determination refers to the measurement of the disappearance of the starch substrate when mixed with amylase in the sample. The addition of L-tartrate measurement of CP activity increases the sensitivuty of the substrate used to the prostatic ACP isoenzyme a. both are true b. both are false c. only second is true d. only first is true