On which part of the progress curve was enzyme activity measured and why?
Q: 40. What role do enzymes play in the cell with regards to chemical reactions? Can they work against…
A: Enzymes are the biological catalysts that accelerate the biochemical reactions. The reactant for the…
Q: Briefly explain the principle of Bradford Method of Total Protein Estimation
A: Asked : Principle of Bradford Method : Total Protein Estimation.
Q: Calculate kcat for an enzyme where Vmax is 12 U s. mL and the total enzyme concentration is 0.016 U…
A: Given Values: Vmax = 12 u s-1ml-1 Total enzyme concentration = 0.016 U ml-1
Q: dentification of the active site of an enzyme: 2.3 Enzyme modification by chemical procedures…
A: 1.The active site is the region of an enzyme where the substrate binds and hence results in…
Q: Name four methods that are used for enzyme immobilization.
A: Hi! Thank you for the question. As you have posted multiple questions, I will be answering the first…
Q: Biosafety level requirement to identify presence or absence of covid-19
A: Covid-19 is caused by corona virus.Corona virus is define as virus which is responsible for…
Q: 75. Which of the following represents a physiologically reversible covalent modification of an…
A: Covalent modifications are modifications done to the structure of the protein that is catalyzed by…
Q: Given the following data in enzyme-catalyzed reaction, what is the Vm, Km and type of inhibition of…
A: Enzyme inhibition refers to decrease in enzyme activity due to certain molecule blocking the active…
Q: What is the difference between saturated and unsaturated fast? what is the difference between mono-…
A: In biology carbohydrates, lipids, proteins, and nucleic acids are four macromolecules, each one of…
Q: 33. Does phosphorylation always increase enzyme activity?
A: The enzyme is a substance found in living beings that works as a catalyst, controlling the rate of…
Q: What are the four key types of irreversible inhibitors that can be used to study enzyme function?
A: An enzyme is defined as a substance that is capable of accelerating the rate of a biochemical…
Q: Which of the following is the Haworth Projection of Glucose?
A: The cyclic structure in Haworth projection displays the ring as flat. In the associated Haworth…
Q: What is the impact of new km value 0.1mM compared to previous km value 0.05mM on affinity of the…
A: Here the graph shows, km value of 0.1mM decreased when compared to 0.05mM. Affinity for the enzyme…
Q: What is lactose intolerance? Discuss diet therapy employed for those experiencing the said…
A: Lactose is the sugar present in milk and dairy supplements. Lactose is a large sugar molecule that…
Q: [Substrate, uM] Vo with DEDS (µM/min) Vo without DEDS (µM/min) 3.333 0.774 1.196 4.000 0.877 1.316…
A: Given data of interest (X, Y) values where X denotes the substrate concentration in uM and Vo…
Q: What are the biochemical responses to a hearty breakfast?
A: Introduction: Breakfast is considered the most important meal of the day and it should not be…
Q: 27. What is the Michaelis – Menten equation and the enzyme kinetic parameters shown in this…
A: Biochemistry involves many reactions which use enzymes. To study these reactions, few equations have…
Q: Explain the Biochemical changes during heat and cold ?
A: The human body maintains the body temperature in a homeostasis manner. The constant body temperature…
Q: Why are DG°' values not rigorously applicable to biochemical systems?
A: Gibb’s free energy is the standard free energy that complements the formation of 1 mole of that…
Q: In glucose estimation using Benedict's Reagent, what is/are the chemical reaction/s that resulted in…
A: Benedict reagent contains potassium thiocyanate, copper sulphate and sodium carbonate. This reagent…
Q: What is the function of the glucose-alanine cycle in normalmetabolism?
A: The glucose-alanine cycle is also known as the alanine cycle or the Cahill cycle. It occurs under…
Q: Determine the Ki for the inhibitor at 30 °Cand decide what type of inhibitor is being used.
A: Michaelis menten constant, Km is the substrate concentration required to produce half maximum…
Q: What is the impact of the lower value Vmax on the affinity for enzyme for substrate? And what is…
A: The relationship between Km and substrate concentration is that Km corresponds to the substrate…
Q: Factors Affecting Enzyme Activity: Catalase
A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…
Q: What do reduce means?
A: Redox reaction is the combination of oxidation and reduction of two molecules participating in that…
Q: What are prodrugs? How is the therapeutic effect of a prodrug affected by enzyme inhibition and…
A: Prodrugs undergo a transformation before exhibiting its pharmacological effects. They comprise…
Q: How do the KM values for glucokinase and hexokinase reflect their roles in sugar metabolism?
A: The Km value is the Michalis constant, which determines the affinity of the enzyme with the inverse…
Q: 4. Why is the type of cell (aerobic/ anaerobic) important to the purpose of this enzyme?
A: Enzymes are highly efficient biological catalysts that speed up metabolism or the chemical reactions…
Q: What is the role of pyridoxal phosphate in transaminase determination? What is the clinical…
A: Transaminases, which are also called aminotransferases, are a group of enzymes that help move an…
Q: If you did not use a saturating concentration of pNPP to establish your initial calibration curve,…
A: p-Nitrophenyl Phosphate (PNPP): It is a non-proteinaceous, non-specific substrate for protein,…
Q: name the enzyme that is used in this reaction and explain why
A: It can be observed that a phosphate group is added to the product so the enzyme must be a Kinase.
Q: What types of substances or activities denature enzymes? Suggest three different denaturation…
A: Enzymes are biocatalysts that are involved in carrying out chemical reactions. They tend to increase…
Q: What Are the Magnitudes of Enzyme-Induced Rate Accelerations?
A: A reaction that is catalyzed by an enzyme, proceeds at a much faster rate because enzymes lower the…
Q: Explain the basis for identification using biochemical testing. – Again, discuss enzymatic pathways,…
A: Biochemical test is the conventional and common method which helps to identify microbes, generally…
Q: Discuss the advantages and disadvantages of Protein efficiency ratio(PER)
A: Protein efficiency ratio (PER) is the weight gained by a test subject divided by its intake of a…
Q: What are the meanings and differences between Ki, Km, and IC50? Are there certain advantages or…
A: Enzymes are biocatalyst. They speed up the rate of biochemical reaction. Enzyme binds with substrate…
Q: DNS reaction is an alternative assay for enzyme activity .What is involved or basis for this…
A: DNS or Dinitrosalicylic acid reaction is used to detect the presence of reducing sugars present in…
Q: 45: Explain about Hunters disease. Which enzyme deficiency causes the disease?
A: Mucins are combination of proteins and carbohydrates. Mucins are also known as mucopolysaccharides.…
Q: PT used in factor 7 deficiency .56 diagnosis F
A: Clotting factors These are circulatory plasma proteins manufactured by liver. Clotting factors…
Q: When considering treatment options for SCID, enzyme replacement offers a permanent fix for patients…
A: Severe Combined Immunodeficiency Diseases It is a condition where the genetic mutation affects the…
Q: Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the ab- sence…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: If the higher value of KM resulting in the new plot ( red curb ) is due to the presence of an enzyme…
A: The concentration of irreversible inhibitor is less than the concentration of enzyme, an…
Q: milk digestion and meat digestion tests are biochemical tests?
A: Biochemical test is defined as a type of traditional method that is used to identify the species of…
Q: sources of error in glucose determination?
A: Glucose monitoring has become an integral part of diabetes care but has some limitations in accuracy…
Q: Discuss the biochemical processes that contribute to maintaining caloric homeostasis
A: Homeostasis can be defined as a state of equilibrium in which the body's internal environment lies…
Q: What effect did high and low temperatures have on enzyme activity? Why?
A: Enzymes are the biological catalyst which increases the rate of reaction without changing itself.…
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- Indicate what will happen (increase, decrease or no effect) to the activity of the enzyme or rate of the metabolic pathway given the following conditions.Q11: **hydrolysis is not the correct answerWhat effects do cooler and warmer temperatures have on enzyme/substrate interactions? Explain. Thank you!