4- You have isolated a peptide, which you wish to sequence. First you treat thepeptide with CNBr and isolate two fragments. In a separate test of the peptide youcleave with trypsin and obtain 3 fragments. You sequence each of the five peptidesand get the following data:Peptide 1: VALKTQAMPeptide 2: LDGKMPeptide 3: TQAMPeptide 4: LDGKPeptide 5: MVALKThe sequence of the original peptide is:

For determination of which of the 20 possible amino acids are present in a particular protein, and…

Answered: Peptide 1: VALKTQAM Peptide 2: LDGKM…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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1. Which peptide would be more soluble at pH 7.0, (Val)₂₀ or (Asp)₂₀ ? at pH 3.0, (Gly-Glu-Val)
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Peptide 1: VALKTQAM Peptide 2: LDGKM Peptide 3: TQAM Peptide 4: LDGK Peptide 5: MVALK The sequence of the original peptide is: