Prepare six different concentrations of Bovine Serum Albumin (BSA) standard from a 2 mg/mL stock. Based on the volumes given, what are the final concentrations of BSA for each standard (1 - 6)? Tube 2.0 mg/ml 0.15 BSA (µl) M Bradford Protein Molarity Dilution A595 NaCl (µl) (mg/tube) from stock Reagent |(ml) 1 100 1.0 2 20 80 1.0 3 40 60 1.0 4 60 40 1.0 5 80 20 1.0 6. 100 1.0 The molecular weight (Mw) for Bovine Serum Albumin (BSA) is 66,430.3 g/mol. Calculate the molarity of BSA in each of your standards based on this value and the mg/mL concentrations you derived above.

Prepare six different concentrations of Bovine Serum Albumin (BSA) standard from a 2 mg/mL stock. Based on the volumes given, what are the final concentrations of BSA for each standard (1 - 6)? Tube 2.0 mg/ml 0.15 BSA (µl) M Bradford Protein Molarity Dilution A595 NaCl (µl) (mg/tube) from stock Reagent |(ml) 1 100 1.0 2 20 80 1.0 3 40 60 1.0 4 60 40 1.0 5 80 20 1.0 6. 100 1.0 The molecular weight (Mw) for Bovine Serum Albumin (BSA) is 66,430.3 g/mol. Calculate the molarity of BSA in each of your standards based on this value and the mg/mL concentrations you derived above.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

ChapterA1: Evaluation Of Analytical Data

Section: Chapter Questions

Problem A1.22QAP

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SCE students measured the concentration of HCl in a solution by titrating with different indicators to find the end point.Indicator Mean HCl concentration (M)(± standard deviation)Number of measurementsBromothymol blue 0.09565 ± 0.00225 28Methyl red 0.08686 ± 0.00098 18Bromocresol green 0.08641 ± 0.00113 29Is the difference between indicators 1 and 2 significant at the 95% confidence level? Answer the same question for indicators 2 and 3
A 50.00 (±0.03) mL portion of an HCl solution required 29.71(±0.03) mL of 0.01963(±0.0030) M Ba(OH)2 to reach an end point with bromocresol green indicator. The molar concentration of the HCl is calculated using the equation below (attached image): a.) Calculate the uncertainty of the result (absolute error). M=0.02333(±?????) M b.) Calculate the coefficient of variation for the result. CV= (Sy/y) x 100%
1. What is the MW of the analyte?2. What is the meq of the analyte?3. What will be the weight (in grams) of the analyte if the standardization is to be quartered?4. What will be Normality of the solution if 7.5ml of the titrant was consumed in the standardization?5. What is the average Normality if two or more trials were conducted with the values of 0.102N and 0.105N?
Standardization of Sodium Thiosulfate Solution Primary Standard used: Potassium Dichromate Formula mass of 1o standard: __________________ % Purity of 1o standard: 99.80% Trials 1 2 3 Weight of K2Cr2O7 (g)Weight of K2Cr2O7 (g) 0.0315 0.0331 0.0380 Final Volume Reading Na2S2O3 (ml) 27.50 27.50 34.90 Initial Volume Reading Na2S2O3 (ml) 1.10 0.00 1.00 Net Volume Na2S2O3 used (ml) Molarity of Na2S2O3
You determine the acetic acid (HOAc) content of vinegar by titrating with a sodium hydroxide standard solution to a phenolphthalein (an indicator) end point. An approximately 5-mL sample of vinegar is weighed on an analytical balance in a weighing boat and this is found to be 5.0268 g. The standard deviation in making a single weighing is 0.2 mg. The sodium hydroxide must be accurately standardised (this means its concentration must be accurately determined) by titrating known mass of high-purity potassium hydrogen phthalate, and three such titrations give molar concentrations of 0.1167, 0.1163 and 0.1164 mol L-1. A volume of 36.78 mL of sodium hydroxide is used to titrate each sample. The standard deviation of the burette used is 0.02 mL. Calculate the percentage of acetic acid in the vinegar and its standard deviation.
If an instrument gives a response of 1240 for a standard containing 8 ppm of a substance, how much if this substance is in a sample that gives a response of 1705? Are any assumptions needed?
help with Part B Please. 5. Compare the values of Keq for each of the five solutions: a. Are any of the values outside the sample standard deviation for the set? b. Does the value of the equilibrium constant change when the concentration of either the reactants or products is altered? Why or why not? Explain this based on your calculations of Keq: c. What caused variation (three reasons) in your values of Keq? NOTE: the standard deviation is 3.9x102 The procedure is in the images Data: Unknown solution Equilibrium concentration of {FeNCS2+} in unknown solution(M) Equilibrium concentration [Fe3+](M) Equilibrium concentration [SCN-](M) Keq 1 3.7x10-5 9.6x10-4 1.6x10-4 2.4x102 2 6.2x10-5 9.4x10-4 3.4x10-4 1.9x102 3 1.1x10-4 8.9x10-4 4.9x10-4 2.5x102 4 1.1x10-4 8.9x10-4 6.9x10-4 1.8x102 5 1.8x10-4 8.2x10-4 8.2x10-4 2.7x102
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The concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…
The thiourea in a 1.455 g sample of organic material was extracted into a dilute sulfuric acid solution and titrated with 37.31 mL of 0.009372 M Hg2+ via reaction: 4(NH2)2CS + Hg2+ →[(NH2)2CS]4 Hg2+ P.S. Answer only the last two letters of the following questions. (Only C and D) a. Is this an example of total analysis technique or concentration technique? Explain. b. Calculate the percent (NH2)2CS ( 76.12 g/mol) in the sample. c. What is classification of the analysis based on the amount of sample and amount of analytes present? Explain. d. If the true value is 10.00%, calculate the absolute and relative error.
A solution of Ca(OH)2 (FM = 74.09) was used in determining the equivalent weight of an unknown acid. To do so, the said solution was standardized against 0.1235 g KHP (FM = 204.22), requiring 7.45 mL to reach the phenolphthalein endpoint. On the other hand, a 0.0543 g unknown acid requires 12.85 mL standard Ca(OH)2 to reach the desired endpoint.a. Calculate the standard normality of Ca(OH)2.b. Calculate the equivalent weight of the unknown acid.c. From the information in the table, what is the most probable identity of the unknown acid?
I would need help with these questions. The method referenced above was followed by a student and she got the following results: Caffeine Std. Conc. (ppm) Absorbance 100 1.806 50 0.899 40 0.724 30 0.545 20 0.365 10 0.183 Further, she analyzed an unknown sample and she got the following results: Sample # Absorbance 1 0.398 1. Graphically find the concentration in ppm and then calculate in mol/L of Caffeine in Sample #1? 2. Which solvent was used to extract Caffeine and why? Why is it necessary to do extraction three times? 3. Cuvettes used in this experiment were made from which material and why? 4. At what wavelength the Absorbance was measured?