Please help even if its just an explanation on how to do this. I am so confused. Thank you so much 3. Using the protein concentrations that you calculated, determine the volume in ul of each samplethat would be needed to load 25ug of that sample on an SDS-PAGE. See example in the first row ofthe table.SAMPLEVolume ofVol of 4xVolume ofFinal totalvolume (uL)sample for25ug (uL)Tris forgelloadingsamplebufferCRUDE8.251750FLOW THRU50PREDESALTING50POST50DESALTING 1. Use the following Absorbance Data to generate a Bradford Assay Standard Curve.Protein ConcentrationAbsorbance at 595nm(mg/mL)0.050.00920.10.020.20.040.30.1120.40.1670.50.19800.60.24362. Use the linear regression equation and the following Abs 595 data and dilution factors forcalculating your protein concentrations:SAMPLEABS 595DILUTIONCalculatedFACTORIprotein](mg/mL)3.098CRUDE0.11410FLOW THRU0.119PRE DESALTING0.1763POST DESALTING0.1573

There are few important points : To determine the amount of protein in an unknown sample is termed…

Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.2 0.02 0.04 0.3 0.112 0.167 0.4 0.5 0.1980 0.6 0.2436 2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 10 FLOW THRU 0.119 0.176 PRE DESALTING 3 POST DESALTING 0.157 3

Protein Concentration Absorbance at 595nm (mg/mL) 0.05 0.0092 0.1 0.2 0.02 0.04 0.3 0.112 0.167 0.4 0.5 0.1980 0.6 0.2436 2. Use the linear regression equation and the following Abs 595 data and dilution factors for calculating your protein concentrations: SAMPLE ABS 595 DILUTION Calculated FACTOR [protein] (mg/mL) 3.098 CRUDE 0.114 10 FLOW THRU 0.119 0.176 PRE DESALTING 3 POST DESALTING 0.157 3

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 8.1P

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