Q1: The concentration of yeast t-RNA in an aqueous solution is 10 M. The absorbance is found to be 0.209 when this solution is placed in a 1.00 cm cuvette and 258 nm radiations are passed through it. a) Calculate the specific absorptivity, including units, of yeast t-RNA. b) What will be the absorbance if the solution is 4 M? c) What will be the absorbance if the path of the original solution is increased to 7.00 cm?

Q1: The concentration of yeast t-RNA in an aqueous solution is 10 M. The absorbance is found to be 0.209 when this solution is placed in a 1.00 cm cuvette and 258 nm radiations are passed through it. a) Calculate the specific absorptivity, including units, of yeast t-RNA. b) What will be the absorbance if the solution is 4 M? c) What will be the absorbance if the path of the original solution is increased to 7.00 cm?

Macroscale and Microscale Organic Experiments

7th Edition

ISBN:9781305577190

Author:Kenneth L. Williamson, Katherine M. Masters

Publisher:Kenneth L. Williamson, Katherine M. Masters

Chapter14: Ultraviolet Spectroscopy, Refractive Indices, And Qualitative Instrumental Organic Analysis

Section: Chapter Questions

Problem 4Q

See similar textbooks

Similar questions

Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.
50 uL of an aqueous sample of double-stranded DNA is dissolved in 950uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What isthe concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wandering as well if the total diluted volume would be 950ul + 50ul = 2000ul
What is the concentration of a tyrosine solution when the absorbance at 280 nm is 1.5 using a 1 cm cuvette and the molar extinction coefficient is 1405 L/mol·cm?
10. a. Light at 278 nm passes through protein RNase A with optical path length is 1μm and the concentration of protein RNase A is 0.5 M. If the absorbance is 0.5, what is the absorptivity in M-1 cm-1? b) What is the corresponding transmittance?
50 uL of an aqueous sample of double-stranded DNA is dissolved in 950uL of water. This diluted solution has a maximal absorbance of 0.326 at 260 nm. What isthe concentration of the original (more concentrated) DNA sample, expressed in ug/ul? I would like to know the process of this question. And I was wondering as well if the total diluted volume would be 950ul + 50ul = 1000ul
If the measured absorbance at 280 nm of the protein in a 1.00-cm cuvette = 0.564, what is the extinction coefficient at this wavelength?
A.) Which parameter between "solvent polarity function" and dielectric constant show better correlation with the peak positions? B.) When a chromophore binds to proteins, the binding site is generally more hydrophobic than the solution. Then, what kind of shift do you expect to observe in the absorption peak position compared to the peak observed when the chromophore is free in solution?
When we perform Bradford Assay to measure protein concentration, the spectrophotometer is set 595 nm because: a. the excitation peak of Coomassie Blue is best observed at 595 nm b. when Coomassie Blue binds to proteins, its maximum absorbance shifts to 595 nm c. the reducing effects of Coomassie Blue shifts the protein's maximum absorbance to 595 nm d. myoglobin is best observed at 595 nm
The absorption coefficient of rhodopsin at 496 nm is 40,000 M-1cm-1. If you have a 1M solution of rhodopsin, how many times will you dilute the solution so that the diluted solution will give you an absorption reading at 496 nm that is in the range of 0.1-1.0. And what will be the absorption by the diluted solution?
the absorbance of an equilibrium mixture of Fescn2+ was measured at 447nm and found to be 0.319. What is the equilibrium concentration of FeSCN2+?
8. a. The value of molar absorptivity constant (e) for a colored substance is 35,000 M -1 cm-1 at 473 nm and the curvette is 1.00 cm. Determine the range of concentration possible if absorbance can be measured from 0.050 to 0.95. b. Calculate the concentration for the following values of absorbance for the colored substance above. A= 0.27 A=0.54
Calculate % transmittance if 85% of light was absorbed by an analyte sample in an UV-Vis spetrophotometric experiment