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- Question 1: Your research project is concerned with the expression and purification of a protein called aldehyde dehydrogenase. Describe in detail all steps required from gene amplification to characterisation of the purified protein.Question:- What is the best description of tRNA (transfer RNA)? Question 15 options: It temporarily stores genetic information. It is an adaptor molecule that translates nucleotide information to polypeptide information. It is the RNA machinery for peptide bond formations. It encodes protein information.QUESTION 23 Which of the following is not part of pre-mRNA processing in eukaryotes? Addition of a 5’ cap Excision of introns Addition of a 3’ poly-A tail Excision of the promoter
- QUESTION NO. 1Targeting a protein to be degraded within proteasomes usually requires ubiquitin. In the function of ubiquitin all of the following are true except: A. ATP is required for activation of ubiquicin. B. a peptide bond forms between the carboxyl terminal of ubiquitin and an ε-amino group of a lysine . C. linkage of a protein to ubiquitin does not always mark it for degradation. D. the N-terminal amino acid is one determinant of selection for degradation. E. ATP is required by the enzyme that transfers the ubiquitin to the protein to be degraded QUESTION NO. 2Much of procollagen formation occurs in the endoplasmic reticulum and Golgi apparatus which requires signal peptide. All of the following statements about targeting a protein for the ER are true except. A. signal peptide usually has a positively charged N-terminus and a stretch of hydrophobic amino acids. B. signal peptide emerging from a free ribosome binds signal recognition…Question 1: tRNA and amino acyl tRNA synthetases Part a: How many codons encode the amino acid Isoleucine (Ile, I)? Part b: How many codons encode the amino acid Valine (Val, V)? Part c: How many codons encode the amino acid Methionine (Met, M)? Part d: How many tRNAs decode the Ile codons? Part e: How many tRNAs decode the Val codons? Part f: How many tRNAs decode the Met codon?QUESTION 15 Which of the following best explains why each codon only codes for a very specific amino acid? The genetic code is not ambiguous The genetic code is redundant, or degenerate There are different reading frames RNA polymerase makes mistakes
- QUESTION 14 Which of the following best explains why there are 64 codons, but only20 amino acids for which they code? The genetic code is not ambiguous The genetic code is redundant, or degenerate There are different reading frames. RNA polymerase makes mistakesQuestion: A gene can best be described as a segment of DNA that A. Transcribed B. Is transcribed as well as the associated regulatory regions C. Encoded for a protein or functional RNA D. Encoded for a protein C. Encoded for a protein as well as the associated regulatory regions Choose the Correct with explanationQUESTION 22 Which type of mutation results in no change in amino acid sequence for the protein? Silent Missense Nonsense Frameshift
- Question 1. Suppose that the diagram below represents the genomic organization of an enzyme involved in eye pigment production in mice. Within the gene are four exons. Biochemical analysis has revealed that the active site of the enzyme is located in the C terminus of the protein. The nucleotide length of each exon and intron is shown. The dinucleotide sequence GT represents the 5’ splice site and the dinucleotide sequence AG represents the 3’ splice site. Both the 5’ and the 3’ splice sites must be present for splicing to occur. Assume that the first and second stop codons are located immediately after the first and second 5’ splice sites, respectively; the third and fourth stop codons are located near the 3’ end of exons 3 and 4, respectively; all these stop codons are in the correct reading frame. E) Suppose you isolate a mutant mouse that has white eyes. When you examine the size of the eye pigment enzyme produced by this mouse, you see that it is 400 amino acids long. Sequence…QUESTION 26 In a double-stranded DNA molecule, how are the sequences of each strand related to each other? A The sequences are identical. B The sequences are identical except that one strand has a U where the other strand has a T. C The sequences are the reverse compliment of each other and use the nucleotides, A, G, U, and C only. D The sequences are the reverse complement of each other.QUESTION 10 Where does translation occur in eukaryotic cells? in the nucleus in the cytoplasm at the golgi apparatus at the plasma membrane