Question: Why are we using this materials in the experiment is there any reason why we using it?Quantification of Total Protein in Serum Experiment;Materials:Fatty acid free beef albuminl solution: 100 g / LReagent ⅠSodium potassium tartrate 9g / LSodium hydroxide 0.2 mol / L Potassium iodide 5 g / LReagent ⅡSerum from blood Copper sulphate solution: 150 g / L Working solution: 5 ml of reagent copper sulphate solution is prepared by mixing into 1 L of reagent Ⅱ. This reagent is stable for 6 months at 2-8 ° CProcedure:*Three test tubes are taken. These are marked as blank, standard and sample tubes.*Only 5 ml of working solution is put into the blank tube.*0.1 ml reagent I and 5 ml working solution are put into the standard tube.*0.1 ml of serum and 5 ml of working solution are put into the sample tube.*After mixing each tube separately, it is waited for 30 minutes at room temperature and measured at 545 nm in the spectrophotometer.*Color permanence is 30 minutes.

Ther spectrometry is the process to detect the quantity of colored complex formed in the reaction.…

Answered: Question: Why are we using this…

Chemistry: The Molecular Science

5th Edition

ISBN:9781285199047

Author:John W. Moore, Conrad L. Stanitski

Publisher:John W. Moore, Conrad L. Stanitski

Chapter13: The Chemistry Of Solutes And Solutions

Section: Chapter Questions

Problem 57QRT

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Question: Why are we using this materials in the experiment is there any reason why we using it?

Quantification of Total Protein in Serum Experiment;

Materials:

Fatty acid free beef albuminl solution: 100 g / L	Reagent Ⅰ
Sodium potassium tartrate 9g / L Sodium hydroxide 0.2 mol / L Potassium iodide 5 g / L	Reagent Ⅱ
Serum from blood
Copper sulphate solution: 150 g / L
Working solution: 5 ml of reagent copper sulphate solution is prepared by mixing into 1 L of reagent Ⅱ. This reagent is stable for 6 months at 2-8 ° C

Procedure:

*Three test tubes are taken. These are marked as blank, standard and sample tubes.
*Only 5 ml of working solution is put into the blank tube.
*0.1 ml reagent I and 5 ml working solution are put into the standard tube.
*0.1 ml of serum and 5 ml of working solution are put into the sample tube.
*After mixing each tube separately, it is waited for 30 minutes at room temperature and measured at 545 nm in the spectrophotometer.
*Color permanence is 30 minutes.

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