ALBUMIN CONCEN- BIURET REAGENT TOTAL STOCK (10 MG/ML) TUBE WATER TRATION ABSORBANCE (A) VOLUME (MG/ML) 1. Blank 5 ml. O mL 3 ml 2. Std. 1 4 mL 1ml 3 ml 0.1 3. Std. 2 3 ml 2 ml 3 ml 0.2 4. Std. 3 2 mL 3 ml 3 ml 0.3 5. Std. 4 1 ml. 4 mL 3 ml 0.4 6. Std. 5 O mL 5 ml 3 mL 1.5 5 ml of unk. + 3 mL of Biuret reagent Absorbance of unk. 7. Unk. Tube = 0.38 A

Appl Of Ms Excel In Analytical Chemistry
2nd Edition
ISBN:9781285686691
Author:Crouch
Publisher:Crouch
Chapter14: Chromatography
Section: Chapter Questions
Problem 2P
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Using the data table below- calculate the concentration of the albumin in each of the test tubes labeled 1 to 6,determine the total volume in each of the test tubes. Prepare a well labeled best fit line graph using the absorbance given, and using the given absorbance of the unknown sample 7 determine the unknown protein concentration. Submit the graph of the data to your instructor electronically following the instructions provided by your instructor.

ALBUMIN
CONCEN-
BIURET
TOTAL
WATER STOCK (10
MG/ML)
TUBE
TRATION
ABSORBANCE (A)
REAGENT
VOLUME
(MG/ML)
1. Blank
5 ml
O mL
3 ml
2. Std. 1
4 mL
1m.
3 mL
0.1
3. Std. 2
3 ml
2 ml
3 ml
0.2
4. Std. 3
2 ml
3 ml
3 ml
0.3
5. Std. 4
1 mL
4 ml
3 ml
0.4
6. Std. 5
O mL
5 mL
3 ml
1.5
5 ml of unk. + 3 mL of
Absorbance of unk.
7. Unk.
Biuret reagent
Tube = 0.38 A
%3D
Transcribed Image Text:ALBUMIN CONCEN- BIURET TOTAL WATER STOCK (10 MG/ML) TUBE TRATION ABSORBANCE (A) REAGENT VOLUME (MG/ML) 1. Blank 5 ml O mL 3 ml 2. Std. 1 4 mL 1m. 3 mL 0.1 3. Std. 2 3 ml 2 ml 3 ml 0.2 4. Std. 3 2 ml 3 ml 3 ml 0.3 5. Std. 4 1 mL 4 ml 3 ml 0.4 6. Std. 5 O mL 5 mL 3 ml 1.5 5 ml of unk. + 3 mL of Absorbance of unk. 7. Unk. Biuret reagent Tube = 0.38 A %3D
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