ALBUMIN CONCEN- BIURET REAGENT TOTAL STOCK (10 MG/ML) TUBE WATER TRATION ABSORBANCE (A) VOLUME (MG/ML) 1. Blank 5 ml. O mL 3 ml 2. Std. 1 4 mL 1ml 3 ml 0.1 3. Std. 2 3 ml 2 ml 3 ml 0.2 4. Std. 3 2 mL 3 ml 3 ml 0.3 5. Std. 4 1 ml. 4 mL 3 ml 0.4 6. Std. 5 O mL 5 ml 3 mL 1.5 5 ml of unk. + 3 mL of Biuret reagent Absorbance of unk. 7. Unk. Tube = 0.38 A
ALBUMIN CONCEN- BIURET REAGENT TOTAL STOCK (10 MG/ML) TUBE WATER TRATION ABSORBANCE (A) VOLUME (MG/ML) 1. Blank 5 ml. O mL 3 ml 2. Std. 1 4 mL 1ml 3 ml 0.1 3. Std. 2 3 ml 2 ml 3 ml 0.2 4. Std. 3 2 mL 3 ml 3 ml 0.3 5. Std. 4 1 ml. 4 mL 3 ml 0.4 6. Std. 5 O mL 5 ml 3 mL 1.5 5 ml of unk. + 3 mL of Biuret reagent Absorbance of unk. 7. Unk. Tube = 0.38 A
Chapter14: Chromatography
Section: Chapter Questions
Problem 2P
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Using the data table below- calculate the concentration of the albumin in each of the test tubes labeled 1 to 6,determine the total volume in each of the test tubes. Prepare a well labeled best fit line graph using the absorbance given, and using the given absorbance of the unknown sample 7 determine the unknown protein concentration. Submit the graph of the data to your instructor electronically following the instructions provided by your instructor.
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