You have been given a mixture of lysine of isoelectric point of 9.74, Histidine of isoelectric point of 7.64 and Cystenie of isoelectric point of 5.02 show how you will separate the mixture into pure forms and describe any instrument that you will use to separate the components in the mixture.

Q: Discuss the structure of phospholipids. Explain the dependence of freezing (gel to liquid crystal…

A: Answer - phospholipids - Phospholipids, are a class of lipids whose molecule has a hydrophilic…

Q: How does the polarity of the amino acid affect its elution within the paper stationary phase?

A:

Q: Imagine you are attempting to separate these three dipeptides from each other using anion exchange…

A:

Q: Please enumerate and explain 1. WHAT ARE THE GENERAL RULES IN GRAMS STAINING CLASSIFICATION OF…

A: In microbiology, Gram staining is the most widely utilised, vital, and widely used differential…

Q: An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated bypaper…

A: As multiple questions are mentioned here, so the solution to the first part is given here. Paper…

Q: Below is the structure of a hypothetical photo-affinity probe analogue of the anti- malarial drug…

A: Cross-links with nearby proteins in the presence of UV light. B is the correct answer.

Q: Fig. 1 shows the possible effect of pH on recovery and impurity removal at the selection of a…

A: The Y-axis of the graph represents the percent recovery of the impurity, and the X-axis of the graph…

Q: The first step in the procedure asks you to prepare five dilutions of the protein standard (BSA) in…

A: During the dilution process, solvent is added to the solution and hence changing the concentration…

Q: When we look at the retention times of the capsaicin and dihydrocapsaicin, they are about a half…

A: The chemical species with higher retention time has greater attraction with the stationary phase…

Q: A researcher wishes to obtain a glycoside (an organic compound) from an aqueous plant extract using…

A: Total amount of glycoside extracted can be calculated using law of distribution. And the molar…

Q: The relative peak areas and retention for a mixture of five fatty acids separated by GC is given in…

A: Which Fatty Acid has the highest percentage? = FA 1

Q: Given a disinfectant concentration dosage of 1.6 mg/L, estimate the retention time that you would…

A: The disinfectant concentration dosage of 1.6 mg/l Chlorine is used as a disinfectant. The…

Q: A mixture of seven amino acids (glycine, glutamate, leucine, lysine, alanine, isoleucine, and…

A: Chromatography is the technique that is used to separate the amino acids based on the priority and…

Q: What is the possible identity of the amino acid? [Select] What is the isoelectric point of AA?…

A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…

Q: The compounds you are separating are; Cholesterol/cholesterol acetate on a silica gel the solvent is…

A: Thin-layered chromatography or gas chromatography system employed which allows a complete separation…

Q: Rank the amino acids used in the experiment in Part II in the order of increasing solubility in…

A: The solubility of amino acids, generally, depending upon the nature of the side chain and they are…

Q: A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas…

A: Because a protein's net charge is pH-dependent, the sample may be eluted from IEX medium by varying…

Q: A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at…

A: Given: concentration of NaCl = 600 mM Since, 1 mL of sample, 600 mM NaCl and HEPES buffer is…

Q: Table 1: Absorbance of Ibuprofen at 221 nm in phosphate buffer (pH 6.8) Concentration of Absorbance…

A: As per Lambert Beer's law, absorbance is directly proportional to concentration of solution.…

Q: Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric…

A: Isoelectric point (pI) is the pH value at which the positive charge equals the negative charge,…

Q: What would be a suitable blank for an assay where the samples contain protein in a solution with dye…

A: A question based on absorption that is to be accomplished.

Q: Biuret Assay accurately quantify protein concentration within the range of 5-150 mg/mL. JUSTIFY THE…

A:

Q: Explain why the LC-MS-MS method is superior to the peptide mass mapping method.

A: Peptide mass fingerprinting (PMF) is an analytical technique for protein identification in which the…

Q: Without performing a calculation, predict whether the standard entropies of the following reactions…

A: Entropy is the measure of randomness in the reaction. If the randomness increases the entropy…

Q: To establish 50% ammonium sulfate saturated solution, 314 g/l of salts should be added. Calculate…

A: It is given in question that solubility of salt is 314 g/L.  This means 314 g of salt is soluble in…

Q: +Protein Mr pI 12,000 28,000 42,000 66,000 (a;) 103,000 (a2) 111,000 А В 8.4 C 7.2 9. E 5 F 4.2…

A: Size exclusion chromatography is an analytical technique that separates the components of a mixture…

Q: The standard curve of a biuret assay gave the equation: y=0.4193x. The absorbance for Unknown A =…

A: Biuret Protein Assay is a colorimetric technique specific to proteins and peptides. Biuret is a…

Q: Given a Ksp of 4.96 X 10-9 for limestone (calcium carbonate), the calcium concentration in a…

A:

Q: which of the following chromatographic methods is best used for separating amino acids?

A: Chromatography is the technique that is used for the separation of different components from the…

Q: For the determination of Pb in blood, a 5.00 mL sample was treated with trichloroacetic acid to…

A: The given data is as follows.      A1 = 0.396 (first point)      A2 = 0.599 (second point)      C1 =…

Q: | This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure…

A: We can find the concentration using the Beer-Lambert law formula which is A = ε*b*C Given that…

Q: Relationship between Affinity and Association & Dissociation Constant. Four proteins (A-D) all bind…

A: Protein with higher value for association constant have higher affinity for ligand. Given data:…

Q: Electrophoresis of a mixture of lysine, histidine, and cysteine is carried out at pH 7.64. Describe…

A: The pH at which amino acid is neutral, or in other words have both negative and positive charge, is…

Q: 3. Isoelectric focusing is a separation technique that takes advantage of the differences in…

A: Isoelectric focusing: It separates the different amino acids based on their differences in their pI…

Q: 1. A vial containing an unknown sample is suspected to be one of the following compounds (mp.): urea…

A: A vial consists of an unknown organic compounds which can be urea, 2,4-dimethylacetanilide,…

Q: Question attached

A: Paper chromatography is a qualitative method in order to detect the presence of colored components.…

Q: When analyzing a PTH (phenylthiohydratoin) derivative of the amino acid isoleucine, resulting from…

A: Reversed-Phase Chromatography: It is a liquid chromatography technique that involves the separation…

Q: what is The method that would be BEST to use to monitor protein secondary structure during the…

A: The secondary structure of the protein is affected by the complex folding properties of the protein…

Q: How does the shape of a titration curve confirm the fact that the pH region of greatest buffering…

A: As there are three question are present I answered only one. you can repost your remaining two…

Q: You were assigned to determine the concentration of an unknown starch solution. [This unknown is…

A: Calculation of concentration (C = A/(L x Ɛ))The Lambert-Beer law, which forms the physical basis…

Q: A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at…

A: Initial concentration of NaCl solution, Minitial = 600 mM Initial volume taken, Vinitial = 1 mL…

Q: Name four factors (bonds or other forces) that contribute to stabilizing the native structure of a…

A:

Q: The pKa values for the α-carboxylic acid and the α-amino acid groups is cysteine are 1.092 and…

A:

Q: Electrophoresis at pH 7.0 of the following lipid mixture is perfo lipid mixture…

A:

Q: The peptide below is placed into a buffer with a pH = 1.54 -NH-CH-C HO- H;N-CH- CH2 -NH -CH-C…

A: We have to find out the direction of movement of peptide at pH=1.54

Q: You would like to calculate the concentration of a protein in a solution measured in a 96 well…

A: