1. please do not worry about the table, answer the questions. thanks.2.images You compare the proteins from blood samples from 2 siblings; one with blond hair and the other with black hair, by 2D gel electrophoresis. What method would you use to identify unique proteins on the gel. Why Step ProcedureCrude extractTotal protein (mg)30,000Specific ActivityActivity (units)6,000,0005,000,0004,000,0003,000,0002,000,0001,800,0001Precipitation (salt)Precipitation (pH)lon-exchange chromatographyAffinity ChromatographySize-exclusion chromatography215,000312,000144000100156From the information in the table, calculate the specific activity of the enzyme after each purification procedure andcomplete the right column of the table. Use this table to answer questions 2-4.102.Which of the purification procedures used for this enzyme is the most effective and why?3.Which of the purification procedures is the least effective and why?4.After step 6, how would you assess if you have pure protein?5.What is the best method for the Covid-19 antigen test? Briefly describe how this method works.

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You compare the proteins from blood samples from 2 siblings; one with blond hair and the other with black hair, by 2D gel electrophoresis. What method would you use to identify unique proteins on the gel. Why

You compare the proteins from blood samples from 2 siblings; one with blond hair and the other with black hair, by 2D gel electrophoresis. What method would you use to identify unique proteins on the gel. Why

Appl Of Ms Excel In Analytical Chemistry

2nd Edition

ISBN:9781285686691

Author:Crouch

Publisher:Crouch

Chapter16: Data Processing With Excel

Section: Chapter Questions

Problem 5P

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Question

1. please do not worry about the table, answer the questions. thanks.

2.images

Step Procedure
Crude extract
Total protein (mg)
30,000
Specific Activity
Activity (units)
6,000,000
5,000,000
4,000,000
3,000,000
2,000,000
1,800,000
1
Precipitation (salt)
Precipitation (pH)
lon-exchange chromatography
Affinity Chromatography
Size-exclusion chromatography
2
15,000
3
12,000
14
4000
100
15
6
From the information in the table, calculate the specific activity of the enzyme after each purification procedure and
complete the right column of the table. Use this table to answer questions 2-4.
10
2.
Which of the purification procedures used for this enzyme is the most effective and why?
3.
Which of the purification procedures is the least effective and why?
4.
After step 6, how would you assess if you have pure protein?
5.
What is the best method for the Covid-19 antigen test? Briefly describe how this method works.

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