Separate a mixture of Vitamin D and Vitamin B2 (riboflavin) by paper chromatography. The stationary phase is methanol (CH;OH) and the mobile phase is pentane (CsH12). Place the vitamins in order of elution. Explain why using IMF. (Circle polar sites on each molecule and label all 4 molecules as polar or non-polar.) Vitamin B HC H,C CH CH, CH H,C CH2 Vitamin D CH,OH
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- in a chromatographic analysis of water from a drinking fountain gives area of 1.56*10^4, 5.12*10^4, 1.49*10^4 and 1.76*10^4 for CH3CL3, CHCL2BR, CHCLBR2 and CHBR3 respectively. determine the percentage concentration of each of the triichoromethane in the sample1. What is the MW of the analyte?2. What is the meq of the analyte?3. What is the weight (in grams) of the analyte?4. What will be the percentage purity of the sample if 43mL of the titrant was consumed?5. Did the sample conform to the USP requirement? Yes or No?Enumerate at least two important things to do to maximize yield when using vacuum filtration in the synthesis of aspirin.
- What is the function of a desiccant in a set-up of volatilization gravimetry? To remove nitrogen from the sample To remove sulfuric acid from the sample To remove water vapor from the sample To remove carbon dioxide from the sampleIn what order should the following methods be used to separate a mixture containing sand-sugar-alcohol and water into its components? 1) Adi distillation II) Fractional distillation HII) Filtration under vacuum B. II, I, II C. III, I, II D. 1, III, II E III, II, IConsider the following statements as they apply to gas chromatography. Decide if each is true or false. 1. ___ Temperature programming is necessary only for samples containing compounds with a narrow range of boiling points. 2. ___ GC carrier gases are generally inert gases like helium and nitrogen. 3. ___ The injection port temperature should be set at the average boiling point of all analytes. 4. ___ An FID is a mass-sensitive detector, not a concentration-sensitive device. 5. ___ An air peak is commonly used to determine the dead time when using an FID detector.
- 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the relative polarities of caffeine, aspartame and benzoate.5) Given the data of the diet sodas, which one would you recommend to be the healthiest? (You may need to research the functions of caffeine, aspartame and benzoate. Please add references if necessary.)1. Are there any difference between using a 2% NaCl and using a water in the paper chromatography. If there are, what is the differences between the two? If they are the just the same, state the reasons.Imagine you are attempting to separate these three dipeptides from each other using anion exchange chromatography, and you use a protein assay to identify at what salt concentration each of them is eluted from the column. A graph showing protein concentration as a function of salt concentration is shown below the dipeptides. Which of the three dipeptides is represented by peak #3? (NOTE: the dipeptides are shown in their non-ionized forms. You must assume that they are in aqueous solution at physiological pH, ~7.2) a) Dipeptide A b) Dipeptide B c) Dipeptide C
- write the principle of gas chromatography? write application of gas chromatography? how it is different from normal chromatographyBased on this. What was the percentage error in the percentage of water in the sample, compared to pure MgSO4•7H2O?4g 5 g 2 g 100 g 49." R Lactic acid Salicylic acid Trichloroacetic acid Flexible collodion qs ad Sig: wart remover. Use as directed. (a) Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 30 g of the mixture? (b) The specific gravity of castor oil is 0.955. How many milliliters of the oil is contained in 30 g of the mixture? (c) If the specific gravity of the mixture is 0.781, what are the percent w/v con- centrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture?