Show calculations for making 10 mls each of the following dilutions of sucrose from the 0.30 M stock solution (Formula #3, Example D) CSTOCK (M) VSTOCK CDESIRED (M) V. DESIRED 0.30 M 0.25 M 0.20 M 0.15 M
Q: In the work-up, the reaction mixture is neutralized with 3M sulfuric acid to a final pH of 1-2. Why…
A: PH of a substance or reaction is defined as the negative logarithm of the hydrogen ion…
Q: You have obtained a 10X fold purification and a yield of 10% at the Affinity step. What was the…
A: Given, Specific activity (SA) at affinity step = 20833 U/mg Total activity (TA) at affinity step =…
Q: 1.1)the following data duscribe an enzyme-catalyzed reaction(hydrolysis of…
A: Background information Km is that substrate concentration at which velocity is half of the maximum.…
Q: Give one test to show the difference between reducing and non-reducing sugars. (Give the indication…
A: Sugars are sweet-tasting compounds. They can be monosaccharides, disaccharides based on the number…
Q: G, L, + and – reactions, please tell me whether you would expect there to be a band in the agarose…
A: Agarose gel electrophoresis is a molecular technique used to separate molecules based on their…
Q: Tris [tris(hydroxymethyl)aminomethane] is a common buffer for studying biological systems. (K, =…
A: pH generally refers to the periodic element and a unit of measurement and the full form is potential…
Q: Substrate Concentration (mol L1) Velocity (mM min-1) 2.500 0.588 1.000 0.500 0.714 0.417…
A: According to Michaelis-Menten Kinetics, when the rate or velocity of an enzyme catalyzed reaction…
Q: Vmax and Km were calculated for enzyme substrate data. Results Y=0,0063x+0,3929 Km =62,4 mikromol/L…
A: From the LB plot of enzyme kinetics, y=mx+c It's an equation of a straight line. 1/V=(Km/Vm )1/S…
Q: the cell potential table found in the Reference Material, why do you get holes in your aluminum…
A: The natural process that results in the conversion of a reactive metal into a stable metal by…
Q: 1. Penicillin is hydrolyzed and thereby rendered inactive penicillinase, an enzyme present in some…
A:
Q: Lineweaver-Burk plots of enzyme kinetics for the reaction, S P, has the following features: 1/v is…
A:
Q: In your acid phosphatase enzyme kinetics lab, you constructed a Lineweaver Burk plot. Lefs assume…
A: Enzymes are biological catalysts that help chemical processes occur faster. Enzymes are proteins…
Q: 1. A Lineweaver-Burk Plot is shown below. 30 25 Curve A y = 3.1207x + 2.4978 20 15 Curve B y =…
A: If an enzyme follows Michaelis-Menten Kinetics, a plot of the reciprocal of the reaction…
Q: calculate the reaction velocity at saturating substrate concentrations. Your numerical answer is…
A: Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…
Q: suctose density gradient ultractifugation is a powerful technique for fractionating macromolecules…
A: The sucrose density gradient is used for fractionating the DNA, RNA, and protein. The samples are…
Q: To purify a given enzyme from a crude extract that contains it, fractional precipitations, exchange…
A: Protein purification is necessary to purify the protein to homogeneity. Homogeneous protein sample…
Q: Q. You have now carried out the Somogyi Nelson determination of reducing sugars and measured the…
A: Principle: In Somogyi Nelson method the reducing sugars are heated with alkaline copper tartrate…
Q: "Bacterial cells are often represented by the chemical formula C5H7NO2. Compute the theoretical…
A: The carbonaceous oxygen demand is also represented as the BOD or the biochemical oxygen demand from…
Q: An amylase result is 550 U/L. A 1:4 dilution is made and reads 180 U/L. What is the reported result?
A: Enzymes act as biological catalysts that fasten reactions and increase the reaction rate by lowering…
Q: Below is a simple set of weights obtained while immersing potato slices in various sugar solutions…
A: Note: As per Bartleby Guidelines For Remaining Answers Please Repost The Question. Introduction:…
Q: Glucose 6-phosphate Glucose 1-phosphate After reactant and product were mixed and allowed to reach…
A: Glucose-6 phosphate is the 1st intermediate of the process of glucose metabolism. It plays a major…
Q: Vmax [S] Km + [S] Vo = Eadie-Hofstee plot V v=Vm-Km [S] Lineweaver-Burk (L-B) plot 1 1 Km 1 Vm Vm…
A: Enzymes increase the rate of a reaction by decreasing the activation energy required for the…
Q: concentration of each enzyme and with [X] = 1 µM. Which curve corresponds to which enzyme? Explain.
A: Given, the substrate concentration is [X] =1 μM Km of enzyme A = 2.0 μM Km of enzyme B = 0.5 μM
Q: A bacterial enzyme catalyzes the hydrolysis of maltose as shown in the reaction given below:…
A: We know by Michaelis–Menten kinetics: V0= Vmax[S]/ Km +[S] V0= velocity of reaction Vmax= maximum…
Q: If photosynthesizing green algae are provided with CO2 containing heavy oxygen (180), which of the…
A: The Calvin cycle is the light-independent chemical reaction in which carbon dioxide is fixed to…
Q: Calculate the equilibrium concentration of H2O for the following esterification reaction performed…
A:
Q: [S] (MM) 0.5 1.0 1.5 Vo No Inhibitor (mM min-¹) Vo With Inhibitor (mM min¹¹) 16.67 25.25 30.49 23.5…
A: Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…
Q: If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02…
A: Enzymes are macromolecular biological catalysts that accelerate chemical reactions. The molecules…
Q: Vmax [S] Km + [S] Vo Eadie-Hofstee plot Lineweaver-Burk (L-B) plot v=Vm-Km [S] Km 1 Vm Vm [S] 1 The…
A: Uncompetitive inhibition is a kind of inhibition in which the inhibitor of the enzyme binds to the…
Q: B) Phosphate buffer is often used to buffer biological systems. Phosphate has three relevant pK,…
A: The given pKa values of the H3PO4 are 2.14, 6.86, and 12.40. pKa value is the pH at which the…
Q: For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much…
A: RBC is the red blood cell and RBC suspension is normally used in serological testing and are usually…
Q: In the Isoelectric Precipitation of Proteins: Casein from Milk - What would happen to the…
A: Casein is a protein, which is present in milk. These are present in the milk in the form of calcium…
Q: An enzyme catalysed reaction has a Km of 8 mM and a Vmax of 13 nM.s-1. Use the Michaelis-Menten…
A:
Q: What type of ion exchange resin and what buffer pH would you use to purify pepsin from an extract of…
A: Chromatography refers to the separation technique that helps in the separation of a compound mixture…
Q: From a kinetics experiment, the Vmax was determined to be 450µM∙min-1. For the kinetic assay, 0.1mL…
A: Kcat is the turnover number of times each enzyme site converts substrate to product per unit time.
Q: Describe how the functional units for beta carotene, xanthophyll, chlorophyll A, and chlorophyll B…
A: Beta carotene is a red-orange colored pigment the is found in red/orange colored plant products like…
Q: Give two advantages and two disadvantages to using the biuret reaction to measure protein…
A: Aromatic amino acids such as tyrosine, tryptophan and phenylalanine exhibit strong UV-light…
Q: Sephadex G-75 has an exclusion limit of80,000 molecular weight for globular proteins. If you tried…
A: Sephadex is a cross-linked dextran gel, which is used for gel filteration. These highly specialized…
Q: A Lineweaver-Burk Plot is shown below. 30 Curve A y = 3.1207x + 2.4978 20 15 Curve B y = 1.0003x +…
A: Lineweaver-Burk plot is a graphical representation used in enzymology. It can be used in the absence…
Q: 1. Enzyme X has a molecular weight of 35,000 Da. When this enzyme is assayed for enzyme activity,…
A: Enzyme kinetics is used to study the rates of enzyme catalysed biochemical reactions. In this study…
Q: Calculate the percentage efficiency of extraction of phycocyanin in sample y, if the amount of…
A: Percentage efficiency of extraction = OutputInput×100
Q: The total energy in linoleic acid is 2,520 kcal/mol and the total amount of ATP that can be…
A: Introduction: Linolenic acid is a type of fatty acid. Linolenate (in the form of esters of linolenic…
Q: The Keq (25C) of the reaction below is 635.67. Fructose 1,6-biphosphate fructose -6-phosphate +…
A: The Gibbs free energy of a system represents the degree of randomness of the system. It is described…
Q: Given that the molar extinction coefficient of lysozyme is 37200 Abs M-1cm-1, calculate the…
A: According to the Lambert Beer's law: Absorbance = (extinction coefficient) x (concentration) x (path…
Q: Table 4. Rates of saponification at 35°C. Ester k(M-' sec=!) k, relative* (CH,O),PO, (CH,O),P=O…
A: Introduction A phosphodiester bond is formed when two of the hydroxyl groups in phosphoric acid…
Q: A 25.0 mL sample of a fruit juice requires 13.0 mL of 0.0100 M I2 solution for reaction. How many…
A: The number of moles of solute dissolves in the volume liter of solution is known as molarity.…
Q: The following chromatogram was the output of the analysis of phytosterols in plants a gas…
A: Asked : Question related to given gas chromatogram
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- Indicate whether each of the following disaccharides is a reducing (R) or nonreducing (NR) sugar by the criterion of reaction with Fehling’s solution. (a) Glcα(1 → 2)Frucβ (b) Galβ(1 → 4)Glc (c) Glcα(1 → 1)Glcα (d) Glcα(1 → 4)Glc (e) Glcβ(1 → 6)GlcSubstrate Concentration (mol L1) Velocity (mM min-1) 2.500 0.588 1.000 0.500 0.714 0.417 0.526 0.370 0.250 0.256 Determine the values of Km and Vmax for the decarboxylation of a 훃-keto acid given the followingdata. You have to plot the graph by using excel and please include the scope of graphDetermine the values of KM and Vmax for the decar-boxylation of a β-keto acid given the following data. Substrate Concentration (mol L1) Velocity (mM min1)2.500 0.5881.000 0.5000.714 0.4170.526 0.3700.250 0.256
- Explain why the combined presence of polyphenol oxidase and iron greatly increase the instability of bulk oils or oil containing foods?Indicate the net charge of cysteine at pH 9.5 Express your answer using two significant figures.If you were presented with 2 L of a 2 M sucrose stock solution, how many grams of sugar would be in a100 mL aliquot? g
- if you have Quinoa Serving size -170 grams Carbohydrates – 109 grams calculate the DV % for these values?Casein, a protein in milk, has a molecular formula C38H57O9N9. Determine the total BOD, carbonaceous BOD, and nitrogenous BOD of casein in units of “grams BOD per gram of casein”calculate the reaction velocity at saturating substrate concentrations. Your numerical answer is assumed to be in units of M sec-1. [S] = 100 mM k1 = 10 sec-1 k2 = 3000 sec-1 k-1 = 20 sec-1 [E]T = 1 \muμM
- Calculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.Define the following terms briefly as they relate to the experiments Cite an example for each using the Fischer/Haworth projection formula a. Aldohexose b. Reducing Sugar c. HemiacetalProvide 5 differences of HDL and LDL and explain each biochemically.