Dear Tutor, Here is one question with two subparts. According to Bartleby Provide Complete Question correct answer. Step by step. Will upvote. Provide Full Question answer. 3. Sphingosine-1-phosphate (SPP) is important for cell survival. The synthesis of SPP fromsphingosine and ATP is catalyzed by the enzyme sphingosine kinase. The velocity of thesphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, astereoisomer of sphingosine that inhibits the enzyme. The results are shown below.[S] (MM)0.51.01.52.53.5Vo No Inhibitor (mM min¹¹) Vo With Inhibitor (mM min¹¹)16.6725.2530.4937.0438.9123.532.236.941.844.0Construct a Lineweaver-Burk plot to answer the following questions:(a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor?(b) What kind of an inhibitor is threo-sphingosine? Explain.

Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…

[S] (MM) 0.5 1.0 1.5 Vo No Inhibitor (mM min-¹) Vo With Inhibitor (mM min¹¹) 16.67 25.25 30.49 23.5 32.2 36.9 41.8 44.0 Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain. 2.5 3.5 37.04 38.91

[S] (MM) 0.5 1.0 1.5 Vo No Inhibitor (mM min-¹) Vo With Inhibitor (mM min¹¹) 16.67 25.25 30.49 23.5 32.2 36.9 41.8 44.0 Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain. 2.5 3.5 37.04 38.91

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter7: Cellular Respiration: Harvesting Chemical Energy

Section: Chapter Questions

Problem 7TYK

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Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. An understanding of the kinetics of the sphingosine kinase reaction may be important in the development of drugs to treat cancer. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below. Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain.
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