A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange resin is suitable to use if the buffer has a pH of 7.0? a. A resin with a quaternary amino group b. A resin with sulfonic acid group c. A resin containing primary aromatic/aliphatic amino group d. A resin that has carboxylic acid group

A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange resin is suitable to use if the buffer has a pH of 7.0? a. A resin with a quaternary amino group b. A resin with sulfonic acid group c. A resin containing primary aromatic/aliphatic amino group d. A resin that has carboxylic acid group

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.2QAP

See similar textbooks

Similar questions

Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatography (d) Use three diagrams to illustrate the separation of three different proteins by thesemethods.
Aliquots of a 0.5 mg/mL standard of BSA were used to construct a standard curve for the Bradford protein assay. The tubes contained the following amounts of the BSA solution: 0, 20, 40, 60, 80, 100 μL. Each sample then had water added to make the final volume 100 μL. The corresponding absorbencies after adding Bradford reagent were the following: 0, 0.05, 0.09, 0.14, 0.19, and 0.22. If you took 20 μL of an unknown and added 80 μL of water, mixed, took 10 μL of the mixture and 90 μL of water and added the Bradford reagent and saw an absorbance of 0.08, what was the protein concentration of the undiluted unknown to one decimal place?
A weak acid, HA, has a pKa of 6.5. If you are utilizing ion-exchange chromatography with an anion exchange resin, what pH range is beneficial to the binding to the stationary phase? And Why?
In silica gel chromatography, which compounds elute first? A-The least polar B-Those with ionic functional groups C-Those with the lowest boiling point D-Those with the highest molecular weight E-The most polar
Explain how we would run a paper chromatography on an amino acid sample (explain the procedure or method). In a paper chromatography of an amino acid, the solvent front traveled 12.0 cm while the amino acid traveled 6.0 cm. What is Rf value of the amino acid?
Briefly explain how cation exchange chromatography can used in protein separation?
You need to separate a mixture of dyes on an agarose gel. You have been provided with 5X TAE (Tris acetate buffer) The electrophoresis tank needs 250 ml of buffer and the gel volume required is 50 ml. Show calculations for making 1X TAE gel running buffer and 1.25% agarose gel for your experiment. Explain how you will prepare the buffer and gel 4m
You are attempting to analyze samples for the presence of opioids using TLC. Use the internet to find a suitable method. Give the stationary phase, mobile phase, and detection technique used in the method and the URL of the website where you found the information.
. An Analytical Chemist is analyzing a protein sample analysis using a light scattering technique. If the formulation of the subject sample has included surfactants or salt additives, which of the following would adequately apply? . The additives would impact the ionic strength of the sample B. The impact of additives influence can be nullified when using a Light Scattering technique. C. The effect of formulation additives would impact the protein sample analysis, and should be considered as a factor when analyzing the protein aggregation using light scattering technique.
2-pentanone has a retention index of 987 on a poly(ethylene glycol) column (also called Carbowax). (a) Between which two straight-chain hydrocarbons is 2-pentanone eluted? (b) An unretained solute is eluted from a certain column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecane (C11H24) is eluted in 17.22 min. What is the retention time of a compound whose retention index is 1 050?
An unretained solute is eluted from a GC column in 1.80 min. Decane (C10H22) is eluted in 15.63 min and undecan (C11H24) is eluted in 17.22 min. What is the retention time (report to 2 decimal places, in minutes) of a compound whose retention index is 1050?
Which statement is true? Statement 1: In a paper chromatography that uses a cellulose-based filter paper as stationary phase and hexane as the mobile phase, non-polar compounds are expected to have higher Rf values than that of polar compounds. Statement 2: In paper chromatography, the highly adsorbed compounds tend to outdistance the weakly adsorbed ones.