show the complete solution and write answers in 3 significant figures. DYE CALCULATED Rf Tartrazine (yellow #5)           ? Sunset yellow (yellow #6)            ? Allura red (red #40)            ? Brilliant blue (blue #1)            ?   M&M's color COMPOSITION Orange          ? Green          ? Blue          ? Brown          ?       Chromatogram before and after:

Q: Based on the information given, what separation technique(s) is/are most likely feasible? Please…

A: The selective boiling and subsequent condensation of a part in a liquid mixture is referred to as…

Q: What are the processes of concentrate samples analysis in Uv-visible spectroscopy?

A: UV-visible spectroscopy is a fast and simple technique that finds the analyte concentration of a…

Q: The reaction in Fohl’s test will cause the formation of what compound? What type of reaction happens…

A: Fohl's test is the biochemical test of proteins for the detection of presence of Aminoacids with…

Q: Using a pipette, add 2 mL of suspended DEAE SEPHAROSE (anion) resin to a chromatographic column.…

A: DEAE Sepharose matrix is used in Anion exchange column. In the given method power of salt strength…

Q: You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution;…

A: It is basically done for counting the  microorganisms like viruses, bacteria etc  from an unknown…

Q: RED ORANGE YELLOW GREEN BLUE BROWN 1 2 3 4 5 6 7 8 9 1

A: Chromatography comes from the Greek word, chroma which means color, and graphy which means to write.…

Q: The following Rf values were computed from a reverse phase chromatography experiment. Arran them…

A: There are different techniques to separate a compound from a mixture of substances. The different…

Q: If we wanted to know how “red” a red liquid solution is, we would use a light transmittance…

A: Medical instrumentation is branch of science which deals with the study of instruments which are…

Q: Four amino acid samples were subjected to a reverse-phase chromatography set up and yielded the…

A: Amino acids differ from each other on the basis of their side-chain properties. Some amino acids are…

Q: What are the mechanisms of samples separation work in Thin layer Chromatography?

A: Chromatography is a physicochemical technique for the division of compound mixtures, in view of the…

Q: The following strip of chromatography paper has been placed in a polar solvent Which pigments the…

A: In chromatographic techniques, including paper chromatography, the lighter molecules will travel the…

Q: What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).

A: Column chromatography is one of the most useful methods for the separation and purification of both…

Q: Given the chromatogram below in a normal phase, which samples would be the most polar? A O Samples C…

A: The process of separation of molecules in normal phase depends on the distribution of particles…

Q: Metric Prefixes Nano-, Micro-, Centi-, and Milli- Match each prefix with its value. Micro 2 Nano 3…

A:

Q: Which of the following compounds will not discharge the brown/orange color of bromine? (Negative…

A: Bromine test is a qualitative test to detect the presence of unsaturation, phenols or anilines. The…

Q: Would you expect a change in column temperature to have an effect on a reversed-phase…

A: Reversed-phase chromatography (RPC) technique use hydrophobic stationary phase to separate…

Q: Which of the following below will show a NEGATIVE result in Huble’s test? Group of answer choices…

A: Lipids are carboxylic groups is attached to a long hydrocarbon chain. On the basis of the presence…

Q: What is the difference between normal and reverse phase chromatography?

A: The main Difference between Normal and reverse phase chromatography is 1) In normal phase…

Q: In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the…

A: Kool aid chromatography  The blue dye, is more non-polar than the red dye and is attracted more…

Q: Given the graph below, what is the value of Vmax? Numeric answer only, no units needed. 20 18 16 14…

A:

Q: write true if the statement if correct and change the " " word/phrase to make it correct…

A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…

Q: eluent strength increase as solvent becomes less polar in reversed-phase chromatography, whereas…

A: Reversed- phase chromatography – uses a non - polar stationary phase. Eluent strength is increased…

Q: Given the chromatogram below in a normal phase, which samples would be the least polar? O Samples B…

A: To compare the relative polarity of the molecules, the retardation factor (Rf) can be used. The Rf…

Q: If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____.…

A: According to the question, If 10 µL of cells in suspension was mixed with 20 µL of a dye called…

Q: In Experiment 7, Isolation of Citrus Oils, the compounds isolated were the monoterpene compounds…

A: Chromatographic techniques are commonly used in chemistry and biochemistry to separate molecules…

Q: You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your…

A: Viable or the live cells can be counted by the instrument called the hemocytometer. These live cells…

Q: What is a Spectrogram

A: Spectrogram is a visual way of viewing the spectrum of frequencies of signal that varies with time.

Q: Given the information provided, please answer the following question: Volume of cell suspension…

A: For setting up the new culture of the cells, the volume of cell suspension required is to be…

Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: When pure hemoglobin (molecular mass of 64,000 Da) is separated by the following methods, what major…

A: Hemoglobin is a tetramer composed of two alpha and two beta subunits. The Molecular weights of the…

Q: GIVE THE SOLUTION AND FORMULA 1.The physician orders an IV with heparin at 700units/hr. You have a…

A: Nurses are usually responsible for administering drugs in a variety of contexts. Nurses can assist…

Q: Section 1: Flame test for Na*, K* and Ca* ions. Your conclusion for each test will be that the metal…

A: The presence  of carbonate in a compound can be tested using a dilute acid. Upon addition of a…

Q: If you were to dilute 1mL of blue dye in 9mL of water, what would your dilution factor be? A.…

A: When a concentrated solution is made less concentrated that it is said to be diluted. Concentration…

Q: Can paper chromatography be used to separate and identify very volatile substances? Explain your…

A: Paper chromatography is a separation technique that is mainly involved in the principle for the…

Q: The wavelength for cobalt (II) chloride is set to 549 nm. Why is this wavelength being used?

A: In uv visible spectroscopy , we measure the absorption of radiation by the sample at a particular…

Q: When determining CFUs for a dilution of the same sample at 10-2 and 10-3 which of the following…

A: CFU/mL means the number of colony forming units present per mL of the sample. In the above case, two…

Q: What is the chemistry principle behind when (treatment: heavy cream) is subjected to room…

A: Heavy cream temperature is important for the physical characteristics of the cream. At room…

Q: Complete the Table Volume capacity and precision a graduated cylinder 50 mL; P=0.1 mL Volume…

A: Diluting a concentration solution: In order to prepare a lower concentration solution from a given…

Q: Explain the color changes observed throughout the peroxide value of oil experiment. Materials: 1.…

A: The unsaturated fat when undergoing oxidation forms peroxide so the peroxide value denotes the…

Q: Based on the data chart below, how far did the solvent travel up the chromatography paper?

A:

Q: Given the chromatogram below in a normal phase, which samples would be the least polar? • Samples B…

A: Chromatography is a technique, used to separate related compounds from a mixture. It is used for the…

Q: Column Chromatography Another name for separation in column chromatography is

A: Another name for seperation in coloumn chromatography is Adsorption column chromatography.…

Q: Given the chromatogram below, which of the following is the possible identity of unknown sample Y?…

A: chromatography Thin layer Chromatography is a technique to separate non-volatile substances  in a…

Q: Milligrams 47.0 milligrams = Multiple Choice O 0.47 decigrams 4.7 centigrams 0.047 grams 0.00047…

A: 47 milligrams or mg quantity of substance  is equal to 47 /1000 gram i.e. , it is 0.047 g of…

Q: How will you know when the endpoint of the titration is reached? Why is it necessary to mix the…

A: Reaction  It is a process in which reactant molecules react to each other and form another molecule…

Q: It was suspected that a particular mixture contained three components: X, Y and Z. To check this,…

A: Thin-layer chromatography is a technique for separating non-volatile mixes from volatile mixtures.…

Q: Which of the following scientists use this X-ray crystallography?

A: X-ray crystallography is the experimental science determining the atomic and molecular structure of…

Q: Why can't we determine Vo, Ve and Vt for affinity chromatography?

A: Chromatographic techniques separate macromolecules based on size, ion exchange, or their affinity…

Q: What happens if you exclude one or more data sets of the peak area ratio of the chromatograph, how…

A: In chromatography technique, the mixture of components are separated based on their retention times.…

Q: Three sugars (Sugar A, Band C) were applied to a line as part of the set-up of a Paper…

A: Paper chromatography is the basic technique to separate dissolved chemical substances from the…