Standard Curve 1.4 1.2 y = 12.235x R = 0.97758 0.8 0.6 0.4 0.2 0.02 0.04 0.06 0.08 0.1 0.12 BSA (mg) What is the amount of protein in a test tube that has an absorbance of 0.23 at 595nm (in mg)? (give the answer to 3 decimal places) Absorbance 595 nm
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- The protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing 10μL of protein with 40μL of water what would be concentrations of the first 3 dilutions?You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).The prescriber ordered oxacillin 500mg IM q6h. The instructions on the 2g vial state to reconstitute the powder with "11.5mL of sterile water for injection, yielding 250mg/1.5mL" Whatis the strength of the reconstituted solution? How many milliliters would you administer?
- Consider the titration of 30.0 mL of 0.0700 M HONH2 (a weak base; Kb = 1.10e-08) with 0.100 M HI. Calculate the pH after the following volumes of titrant have been added: (a) 0.0 mLpH = (b) 5.3 mLpH = (c) 10.5 mLpH = (d) 15.8 mLpH = (e) 21.0 mLpH = (f) 27.3 mLpH =How many moles of KBr are there in 89.5 mL of 0.150 M KBr?What volume of 2mg/mL BSA stock is needed to make 40uL of 100ug/mL BSA? Group of answer choices A) 2000uL b) 38uL c) 2uL D) 5uL E) 0.8uL
- Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of the original suspension if the thousand-fold dilution of that suspension has an absorbance of 0.3 at 600nm? * A. 3.7 x 10^12 B. 3.7 x 10^9 C. 3.7 x 10^1 D. None of these is correctif you have Quinoa Serving size -170 grams Carbohydrates – 109 grams calculate the DV % for these values?You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
- The doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show workIf the average molar mass of a sample of soybean oil is 1500 g/mol, how many grams of NaOH are needed to saponify 5.0 g of the oil?pH 3.5 4.5 5.5 6.5 7.5 8.5 Absorbance 0.098 0.027 0.068 0.028 0.032 0.054 Concentration in diluted supernatant (mg/ml) 0.196 0.054 0.136 0.0056 0.0064 0.0108 Concentration in undiluted supernatant (mg/ml) 0.98 0.27 0.68 0.28 0.32 0.54 Formula for the amount (g) of soluble proteins in the soy flour extract: In 15 ml of soy flour extract (with 1/50 dilution), Soluble protein (g) = (C x V x F) / 1000 C = concentration (mg/ml); F = dilution factor; V = volume of solution (ml) Calculate the % solubility of protein in the soy flour (Assume the soy flour contains 35% protein (w/w))