Please answer this question. You obtained the following raw data when setting up a Bradford standard curve:BSA (mg/ml)012345678910Absorbancy595nm0.2250.3100.4200.5100.6100.7200.8100.9150.9500.9800.990After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.540 was obtained.Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).

The biuret assay can be used for quantitative determination of total protein concentration by…

Answered: You obtained the following raw data…

Nutrition Through The Life Cycle

7th Edition

ISBN:9781337919333

Author:Brown, Judith E.

Publisher:Brown, Judith E.

Chapter4: Nutrition During Pregnancy

Section: Chapter Questions

Problem 23RQ

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You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 After blanking against a bradford-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.570 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.262 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
Using the data from the standard curve done previously, you record the absorbance of your extracted milk protein sample at 0.499 at 540nm. What is the mass of the protein in mg assuming you collected 10mL worth of the protein? Your standard curve should have a line of best fit with the equation y=0.0001x+0.0038.
Using the equation for the best-fit straight line through your data, the average absorbance of your unknown samples U1 and U2, and any dilution factors (DON’T LEAVE OUT THE DILUTION FACTOR), calculate the concentration of protein in the original unknown protein sample. y = 1.6849x + 0.0414R² = 0.9904
Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______ ul
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH
You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0 0.225 1 0.310 2 0.420 3 0.510 4 0.610 5 0.720 6 0.810 7 0.915 8 0.950 9 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) Answer Skip Exit
If you want to make 7 L of 1X TGS (Tris/Glycine/SDS Running Buffer) using 8 x TGS stock, how much of the 8 x stock do you need? Report your answer to 1 decimal place.
The non linear part of the Standard curve graph can be used for estimating the unknown sample protein concentration? True False
Viscosity measurements were done on native form of Protein XYZ and on 4 denaturants. The crude Protein XYZ was diluted to produce 5% w/v. Using the Ostwald viscometer, the following data were obtained: time, sec time, sec time, sec time, sec Denaturant Blank (Native) Native Blank (Denaturant) Denatured pH= 2.00 70 95 70 101 Temp (90 °C) 70 98 70 150 0.1M Urea 72 97 88 110 DTT 71 96 90 170 a. Calculate and tabulate the reduced viscosities (in mL/g) of the native and denatured Protein XYZ. b. Determine two most effective denaturants. c. What can be inferred with the two most effective denaturants? Dont reject if you dont know the answer!
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.

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