State two reasons behind using the blocking buffer in ELISA experiment.
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State two reasons behind using the blocking buffer in ELISA experiment.
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- Why is a positive and a negative control used in a Elisa experiment?The elimination of several steps in the ELISA could be accomplished if the primary antibody was made into an enzyme conjugate. Why is this generally not done? What can cause a false positive in an ELISA?In relation to ELISA technique, fill in the missing information in the figure below: 1- 2- 3- 4-
- Is there any way to stabilize color after adding stop solution in ELISA in Which HRP and TMB used as enzyme and chromophore?Explain how the competitive ELISA works and what it is used for?If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used.
- What is the role of the substrate? Is it really necessary to add substrates in ELISA? Why, or why not.Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?Describe the principles behind direct and indirect fluorescent antibodytests.