Suppose when you are performing a Gram stain procedure, you hav reversed the order of crystal violet and safranin, you stained with saf irst then, iodine, then wash with alcohol and then with crystal violet. colors would (a) Escherichia coli, and (b) Staphylococcus epidermidi Provide a short explanation for your expected outcome

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing
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I posted this question same person answered twice and I don’t understand the handwriting so plz if same person gonna answers then plz type don’t post same answer over again
T-Mobile Wi-Fi
7:07 PM
O 87%
Step1
a)
E. Coli Chram -ve Bacteria)
Stain Red o pink due to retaining Tme colour
Staining dye called sufanin.
Ans-
Aprer decoloizarlon , tne Gram tue ·cells remains perpė
and . Grem -ve cells
Stein, which Is cusuolly pooitively' changed sufonmin.
or basic fachehe, is apptied last to ve
decolorized
(olour.
loses 8 its puople cotour, Lamper
Cram -ve bactena - 9 pink or red
Step2
b)
O Staphy lo coccus. c Grum tue balteria)
struin dark purple dne to re taining The primny
dye called insrall cidlet in the cell cwall.
Crustal violet is ablue. mono phloide salt g coural
(ahion. The
tue ions g The dye.9n teruet costy
-vely changed. ele ments 'g the cel wald such as
DMA , peptidoglylan and Eipo pelysacchnjdes , thus,
de tai nny The pumple colar 7 the dye . cretal
nolet is qlso a mitoi ont and mutagen
Gram -ve Bactena are
because Their cell wal is impenetrabe.
more resistant ageinst anb bodies
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Transcribed Image Text:T-Mobile Wi-Fi 7:07 PM O 87% Step1 a) E. Coli Chram -ve Bacteria) Stain Red o pink due to retaining Tme colour Staining dye called sufanin. Ans- Aprer decoloizarlon , tne Gram tue ·cells remains perpė and . Grem -ve cells Stein, which Is cusuolly pooitively' changed sufonmin. or basic fachehe, is apptied last to ve decolorized (olour. loses 8 its puople cotour, Lamper Cram -ve bactena - 9 pink or red Step2 b) O Staphy lo coccus. c Grum tue balteria) struin dark purple dne to re taining The primny dye called insrall cidlet in the cell cwall. Crustal violet is ablue. mono phloide salt g coural (ahion. The tue ions g The dye.9n teruet costy -vely changed. ele ments 'g the cel wald such as DMA , peptidoglylan and Eipo pelysacchnjdes , thus, de tai nny The pumple colar 7 the dye . cretal nolet is qlso a mitoi ont and mutagen Gram -ve Bactena are because Their cell wal is impenetrabe. more resistant ageinst anb bodies Was this solution helpful? ?
Suppose when you are performing a Gram stain procedure, you have
reversed the order of crystal violet and safranin, you stained with safranin
first then, iodine, then wash with alcohol and then with crystal violet. What
colors would (a) Escherichia coli, and (b) Staphylococcus epidermidis be?
Provide a short explanation for your expected outcome.
Transcribed Image Text:Suppose when you are performing a Gram stain procedure, you have reversed the order of crystal violet and safranin, you stained with safranin first then, iodine, then wash with alcohol and then with crystal violet. What colors would (a) Escherichia coli, and (b) Staphylococcus epidermidis be? Provide a short explanation for your expected outcome.
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ISBN:
9781133893943
Author:
ESTRIDGE
Publisher:
Cengage