Suppose you are characterizing the DNA of a diploidplant species that had never been analyzed previously.You purify all the DNA that can be isolated from a seedling, and subject this DNA to high-throughput sequencinginvolving millions of reads of random DNA fragments.a. If you obtained on average 100 reads of a givensingle-copy nuclear DNA sequence, about howmany reads would you obtain for mtDNA? ForcpDNA? (Assume each mitochondrion has10 genome copies and that each choloroplast has20 genome copies. Assume also that the averagecell of this plant species has 1000 mitochondriaand 50 chloroplasts.)b. Beyond the number of reads, what other criteriawould allow you to conclude whether a particularread was of nuclear DNA, mtDNA, or cpDNA?

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Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.