Suppose you have just determined the DNA base sequence foran especially strong promoter in Escherichia coli and you areinterested in incorporating this sequence into an expressionvector. Describe the steps you would use. What precautionsare necessary to be sure that this promoter actually works asexpected in its new location?

Q: If you cloned GFP under the control of the V. fischeri Lux promoter, when would you see GFP…

A: Vibrio fischeri exhibits bioluminescence only when the density of its cell is higher and…

Q: The following diagram represents one of the Christmas-tree-like structures shown in Figure  On the…

A: DNA molecule is composed of two polynucleotide chains and coil in a double helix. The molecule…

Q: You have isolated a protein that binds to DNA in theregion upstream of the promoter sequence of the…

A: Positive regulation refers to the increased transcription of a gene by binding of a protein or a…

Q: The lac repressor and the pur repressor are homologous proteins with very similar three-dimensional…

A: The lac represser supermolecule, LacI, prevents the transcription of genes concerned in lactose…

Q: Suppose that you have isolated a mutant strain of E. coli inwhich the lac operon is constitutively…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: The genes that encode the enzymes for arginine biosynthesis are located at several positions around…

A: A gene is a stretch of nucleotides present on a chromosome. It is the region that encodes the…

Q: Explain why the glucocorticoid receptor binds next to the core promoter of some genes, but not next…

A: Gene expression is a process in which the information present in the particular sequences is…

Q: . a. Mice engineered to block expression of the geneencoding the telomerase protein age at a…

A: Telomerase is an enzyme that is essential for the replication of chromosomal ends. It is a…

Q: . One mechanism by which antisense RNAs act as negative regulators of gene expression is by base…

A: The antisense RNA is a type of single-stranded RNA molecule that binds to the complementary…

Q: Another class of suppressor mutations, not describedin the chapter, are mutations that suppress…

A: A missense mutation is a type of mutation in which the error occurs in a single base. It is also…

Q: In the galactose operon of E. coli, a repressor, encoded by the galR gene, binds to an operator…

A: The galactose operon of E.coli is called as gal operon. This operon consists of 4 structural genes.…

Q: True or False: Reoressors regulate gene expression by helping RNA polymerase bind to the promoter.

A: Gene expression is the process by which information from a gene is used in the synthesis of a…

Q: low birthweight (LBW) and normal birthweight (NBW) individuals by treating isolated DNA from both…

A: All cells in an organism contain the same DNA , cell types and functions differ due to the…

Q: Describe and explain the 3-D structural change to the protein (uniprotkb-p39086/gene regulator 5)…

A: Answer-- 3-D structure of proteins is the level of protein structure at which an entire polypeptide…

Q: Recombinant expression in prokaryotic systems has numerous advantages when compared to eukaryotic…

A: DNA is the genetic material found in organisms. Recombinant DNA technology is the isolation of a…

Q: a. The original constitutive operator mutations in thelac operon were all base changes in o1. Why…

A: The original constitutive operator mutations made in the lac operon involved base changes in the O1.…

Q: An electrophoretic mobility shift assay can be used to study the bindingof proteins to a segment of…

A: RNA Polymerase II used in transcription process and produces mRNA, but this enzyme do not act alone,…

Q: A more thoughtful analysis of NANOG expression should consider all post-transcriptional and…

A: Medical experts continue to find new medications that aid in the treatment of deadly diseases and…

Q: You are interested in finding out in which organ GTF2H5 is highly expressed experimentally. Describe…

A: Recombinant DNA also written as rDNA is formed by joining or combining the DNA from different…

Q: Although a large number of mutagenic chemicals are known,none is known that induces mutations in…

A: Mutation is the process of abrupt changes in the nucleotide sequence or gene in the DNA. Mutation…

Q: Consider a mutation in -10 BOX promoter consensus sequence (TATAAT) in the prokaryote recA gene…

A: TATTAT is called pribnow box in prokaryotes and is a promoter sequence that tells the Enzymes where…

Q: Explain the features of the Initiator (Inr) elements, BREs, DPEs, and MTEs of focused promoters.

A: BASIC INFORMATION  TRANSCRIPTION  it is responsible for the formation of hnRNA which has the codes…

Q: what is the AT-rich promoter element located approximately 25-30 bases upstream of the transcription…

A: The promoter is a regulatory region of DNA located upstream (towards the 5' region), the binding…

Q: Mouse models for human genetic diseases are potentially powerful tools to help geneticists…

A: Various types of mouse models have been developed by researchers or scientists that act as very…

Q: The following statement occurs early in this chapter:“. . . a crucial step in the regulation of many…

A: Transcription is a process in which a sequence of DNA is transcribed into mRNA. In bacterial…

Q: You are studying an operon containing three genesthat are cotranscribed in the order hupF, hupH,…

A: Operon is a set of genes which are responsible for conduction of related functions. it is a feature…

Q: . Using a cDNA library, you isolated two differentcDNA clones that have sequences indicating that…

A: A complementary DNA library (cDNA) represents the genes collection that is encoded into the protein…

Q: sulators can block the effects of enhancers only when they lie upstream of a promoter within the…

A: Genetics is the branch of biology that deals with the study of genes, their inheritance patterns,…

Q: What is the role of arabinose in production of GFP protein from pGLO-transformed bacteria? O When…

A: Given: The role of arabinose in production of GFP protein from pGLO transformed bacteria.

Q: A significant number of mutations in the HBB gene that causehuman β thalassemia occur within introns…

A: A mutation occurs when the sequence of DNA changes. Mutations can occur as a result of DNA copying…

Q: When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring…

A: In knockout animal, a targeting vector having a gene of interest is introduced into the cells. The…

Q: You have synthesized an enhancerless GFP reportergene in which the jellyfish GFP cDNA is…

A: The enhancer sequences are regulatory DNA sequences. These sequences when bound by specific proteins…

Q: PROTEIN X HAS THE POTENTIAL FOR MEDICAL APPLICATION IN HUMAN. IT IS ORIGINALLY FROM A  PLANT AND HAD…

A: Genetic engineering is the process of making manipulation in genes and DNA  to get desired traits…

Q: What would you expect krox20 expression to look like in embryos treated with an RA inhibitor? Why?…

A: krox20 is also known as early growth response protein 2, it is a transcription regulatory factor.it…

Q: What conservation laws (P- or S- invariants) can be found in the lac operon regulatory Petri net?

A: The lactose operon or lac operon is an operon that is required for the transport and metabolism of…

Q: Mouse models for human genetic diseases are potentially powerful tools to help geneticists…

A: The mouse is used in the alteration in the genome through the use of genetic engineering technique…

Q: For the following types of transcriptional control, indicate whether the protein produced by the…

A: Operon is a functional unit of DNA that consists of cluster of genes under control of promoter. Gene…

Q: A strain of Arabidopsis thaliana possesses a mutation in the APETALA2 gene. As a result of this…

A: Ribonucleic acid (RNA) is a biomolecule that plays important role in coding, decoding, and…

Q: Both eukaryotic and prokaryotic promoters O contain enhancer or silencer sequences. O contain TATA…

A: A promoter is a region of DNA where transcription of a gene is initiated. Promoters are a vital…

Q: One complication of making a transgenic animal is that thetransgene might integrate at random into…

A: An organism whose DNA has undergone artificial change is referred to as a transgenic organism. This…

Q: ou want to clone the yeast ABC gene into an expression vector. Is it possible to induce the rotein…

A: Transgenic organisms are the organisms which contain a foreign DNA. While cloning eukaryotic genes…

Q: What is the frequency of loss-of-function mutations in ADE2 when specific (HDR) and non-specific…

A: Each pathway, including the non-homologous end joining (NHEJ), resulted in a 50% reduction in…

Q: What properties demonstrate that the lac repressor is a protein?Describe the evidence that it indeed…

A: Lac operon is negatively regulated by repressor protein.

Q: Consider this strain of E. coli with lac operon alleles: IS pt o+ z+ Y+ /I p* oCz- Y+ In a written…

A: Lac Operon is the segment of DNA which includes regulatory gene, promoter gene, operator gene and…

Q: You identify a proflavin-generated allele of a genethat produces a 110-amino acid polypeptide…

A: The chemical name of proflavin is acridine-3, 6-diamine and acts as an intercalating agent.…

Q: In the laboratory, you want to study protein that is normally toxic to E. coli cells.  You wish to…

A: AraBAD promotor is a structural gene of L-arabinose operon in E.coli.  AraBAD basically are 3…

Q: a. Very few if any eukaryotic genes contain tractswith more than 25 As or Ts in a row, yet almost…

A: The process in which the polymerization of ribonucleotides, guided by complementary base pairing…